RnoINT0064445 @ rn6
Intron Retention
Gene
ENSRNOG00000061527 | Gck
Description
glucokinase [Source:RGD Symbol;Acc:2670]
Coordinates
chr14:86152894-86155162:-
Coord C1 exon
chr14:86155063-86155162
Coord A exon
chr14:86153078-86155062
Coord C2 exon
chr14:86152894-86153077
Length
1985 bp
Sequences
Splice sites
5' ss Seq
TGGGTAAGG
5' ss Score
9.08
3' ss Seq
TGCCCCATCCCTCCCTCTAGGCA
3' ss Score
10.67
Exon sequences
Seq C1 exon
GACTTTGAGATGGATGTGGTGGCAATGGTGAACGACACAGTGGCCACAATGATCTCCTGCTACTATGAAGACCGCCAATGTGAGGTCGGCATGATTGTGG
Seq A exon
GTAAGGGCTTCTCCCCCTGGCAACCATTCTAGGCTGCACAAGGGTCATAGCAGCCAATGCCGAAGCATTTACAAGTGCCAGTCAGAACAGGCCTGGTCCCTGAGCCAGGCGGCCGTGGGGGTGTGGTGATATGGGAACTGGAGGTTGATAGGGAGTAGTATGTCAACGTTTGCAGCATCAGGGCGTTAGATGGCCTGCCAGCTGCATGCACACCTGCCCTGCCTATCTGGATGTAATTAAACCGCCTTGTCTGGTAAATTGACCACACTGTACGAATCTGTCCAAGTGAGAGAGAATCGGCAGAAGTGGTACCATCCATGTGGCTGGCAGCCCTGCCTCAGCCCGGCCCTGCCCCTGACAGCAAACCCAGCACTATGAACTGCAGGTCCTAGACCCGCTCTGGAGGGAAGGAGAGTCTTGAAAAGGAAGCCACAGTGCTCTCTCTTAAGCAGGAGCAGGAGCTGCATAGCAACTTCCTCCTCCTGCCCCTGCTCCCACTCTCGGAAGTTCCTGCGTTCTTCCGAAGCAGTAGTTCAACCGAATCAGTTATCTGCTCTGCACACAGAGGTAGAAATGTACGTTTGGACGCTTTGGGCCCTGATTGCCCAGAAAATCCATGTGTTAGGCTTTCCAGAGCCAGATCCCCAATGCCGTGTGTTTGTATTCATAAGTTCTTATCCGTACTTACGCTTGTGCCCTAAATAACATGTTTTATAAACTCCATTCATTTCATATTCATACATATATAAAACGTCATAGGTGTGCACGTACGGGTACACACATATACATATTTACATTTGATATGGCTGTTCTGAGACAGAGTCTCGCTACGTAGCTCTCCCTATCCTAGAACTCACTATGCGGACCAGGCCTCAAACTCACAGAGATCCACTTGCCTCAGCCTCCTGAGTGTTAAGACTAAAGGTATGTGCCACCACACCCGGCTATGTGCTTTACACTCCAACCCGACCCCTCCGCGTCACTTCTCATTTCCTGTTTTTACTTGTGATCTAGCCTGTGTAGTGAAAATCCCTCTCTCCAGATGCCCTTGGTTGTGCCTGCCTCTGCGTCTCCTCCTGAACGCCTCCTGGGGCTCTCAAGACATTTTTTTTTTTGGTTCTTTTTTTTTCCGGAGCTGGGGACCGAACCCAGGGCCTTGCGCTTCCTAGGCAAGCGCTCTCCCACTGAGCTAAATCCACAACCCCTCCCAAGACATTTTAATCTGAACTTAGTCCAGTCACCTATGGCTACTCCCCAGGCACACCGCCACCTCCTGTGGCATGATGCACACGTGATGGGTATCTTTGCTGGCCCATGGCTAAGTGCAGCAGGTCCTGCCTGCAGTAGCTTCTGGAGGGCTTACATGGTGCCTCACTCACTTTGAGAGTCTTCTAAAAGAGTTCAGGAGAGAGACTCCAAAAAGTGCCTGCAACATGCTAGAAAACAAGGGGCTGGAGAGATGGCCGAGGAGTTATGAGCACTTGATGCTCTTCCAGAGGACCTGGGTTTGATTCCCAGCACCCACATGGTGGTGACTCACAACTGTCTGTAACTGCAGTCCCAGGAGATCTATACCCTCCGCTGGCTTCTCTAGGCATCAGACAGACAGACATGTAGTGTGCAGACAGAACATTTATATACCTAACATTTAAAAAAATTTAAAAAGGAGGTTGGGGATTTAGCTCAGCGGTAGAGCACTTGCTAGCAAGTGCAAGGCCCTGGGTTCGGTCCCCAGCTCCGAAAAAAAAGAAGAAGAAAAAAAAAAAGCCATGACCCCCTCACACTCATTTCCAGTGTCAAAACCACATCAGCACACCTTGTTTTTCCTCCATCCCCAGACTTCCCAGGAAAGGAGTGGGGATGCCATTCTTGTTAGAGGGCAGTCTGCAGACTGACCCCGCTACTCTTCGTTACATAGATGCTCCAGTCTCCCAGCATCCACCCCCGGTTCTCACAGCCTCAATGTGCCCCATCCCTCCCTCTAG
Seq C2 exon
GCACTGGCTGCAATGCCTGCTACATGGAGGAAATGCAGAATGTGGAGCTGGTGGAAGGGGATGAGGGACGCATGTGCGTCAACACGGAGTGGGGCGCCTTCGGGGACTCGGGCGAGCTGGATGAGTTCCTACTGGAGTATGACCGGATGGTGGATGAAAGCTCAGCGAACCCCGGTCAGCAGCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000061527:ENSRNOT00000080147:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0034916=Hexokinase_1=PD(14.9=67.6),PF0372711=Hexokinase_2=PU(3.3=23.5)
A:
NA
C2:
PF0372711=Hexokinase_2=FE(25.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTTTGAGATGGATGTGGTGGC
R:
CTGACCGGGGTTCGCTGAG
Band lengths:
278-2263
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]