RnoINT0064446 @ rn6
Intron Retention
Gene
ENSRNOG00000061527 | Gck
Description
glucokinase [Source:RGD Symbol;Acc:2670]
Coordinates
chr14:86151605-86153077:-
Coord C1 exon
chr14:86152894-86153077
Coord A exon
chr14:86151761-86152893
Coord C2 exon
chr14:86151605-86151760
Length
1133 bp
Sequences
Splice sites
5' ss Seq
GCTGTAAGG
5' ss Score
4.86
3' ss Seq
ACCCACGGTTCTGTATCCAGGTA
3' ss Score
9
Exon sequences
Seq C1 exon
GCACTGGCTGCAATGCCTGCTACATGGAGGAAATGCAGAATGTGGAGCTGGTGGAAGGGGATGAGGGACGCATGTGCGTCAACACGGAGTGGGGCGCCTTCGGGGACTCGGGCGAGCTGGATGAGTTCCTACTGGAGTATGACCGGATGGTGGATGAAAGCTCAGCGAACCCCGGTCAGCAGCT
Seq A exon
GTAAGGATGCTCCCTGCCCTCCTAGCCCACCCCGGCAGCTGCTCTGGCCGGGTTCATCACAGCTGGCTCACAGCACACAATAGCTTGTTTATAAGCAGCTCTGGGGAGAAGCAAATTGACGTCCCTCTGTGAGCTGGCTCTTTCCTTCAGCTCAGTTACACCCTGCTCTGTGAGGTGTGGAGCTGTCACGGGGGTTGGTATAAAATCCTGTCCCTCTACTCTCATAGGTGGCCGGCCACTAGATGGCCCTGAAAGACCCTTAGCAAGGTTTATCTGGTTTAAGGCAGAAACCCCCACAGCACATAATGCATGCCAGTTCTACACAATGAGTATTGGCATTTAGGGGAATGTATCCTGGGACCCAACTGTCTTGAATCATGGCTACTGCAGGGTGTTGAGGTATGCTCCTCTAACCTCAACACTCGGGAGGCAGAGTCCCACAAATTGCTTTGTGTTCCAGGCCAGCCAGGGCTACAGAGCAAGTTCCAGAATAGCCAGGGATACATAGTGAGACCCAGTCTCAAAAAATAAAAGTAACTAAGTAAATAAAATGAATGGTGCCTGAAACAAGTTAGCCACCTACATGGTACTGTTCTCACGCTAACTACTTAATGAGTTTCATATCAGAACCTCACAAGCCTCTGTTGGATCATTTAATATCTGCTGTATTGCAAGGTGAAAATATGGAGGCTCACACATTAGACAAAAATATACCAGGCTGCCATGGGCCATGTTGGCACCAACCCTGTGAGCCACTGAGAATAGCTATCATCCCTTCACGTGGTTGTCACTGAATTCCCAGGGTGCGGGCTTCACACAAGGCTTGCCACCAGTAAGCTTTCAAAGGACCACAGAGGCTCCTTAACTCTGTGACCCTTTCATATACCAGGGCCAGCCCAGAGTCAGATGGGGCCCTCAGCTGGGTTTAAAATATGCAAGTTCCTAGGACCTGAGGTTTCTGAAGCCTCTGTTTACAAATGTGGGGGCAGGGTGTGAGGGCAAGGACCTTGGATAATGACTAGGGGGTAGGAATGAGAGGAATCACAGTGTCCCACGTGGTCACCTCCTAACCAGAATAGGCCTGTGGGGATTTCCCGACGGCCTGCTGGCATGACCCACGGTTCTGTATCCAG
Seq C2 exon
GTACGAGAAGATCATCGGTGGGAAGTATATGGGCGAGCTGGTACGACTTGTGCTGCTTAAGCTGGTGGACGAGAACCTTCTGTTCCACGGAGAGGCCTCGGAGCAGCTGCGCACGCGTGGTGCTTTTGAGACCCGTTTCGTGTCACAAGTGGAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000061527:ENSRNOT00000080147:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0372711=Hexokinase_2=FE(25.4=100)
A:
NA
C2:
PF0372711=Hexokinase_2=FE(21.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCTACATGGAGGAAATGCAGA
R:
TCCACTTGTGACACGAAACGG
Band lengths:
320-1453
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]