RnoINT0091340 @ rn6
Intron Retention
Gene
ENSRNOG00000010634 | Megf11
Description
multiple EGF-like-domains 11 [Source:RGD Symbol;Acc:1582797]
Coordinates
chr8:70170521-70172172:+
Coord C1 exon
chr8:70170521-70170649
Coord A exon
chr8:70170650-70171935
Coord C2 exon
chr8:70171936-70172172
Length
1286 bp
Sequences
Splice sites
5' ss Seq
AAGGTAACA
5' ss Score
8.92
3' ss Seq
AGGCCTCCCTCTGCCCACAGCTG
3' ss Score
10.04
Exon sequences
Seq C1 exon
GATGTGCCCCTGGAACCTTTGGATACGGGTGTCAGCAGCTATGTGAGTGCATGAACAATGCCACTTGTGACCATGTCACTGGTACCTGTTACTGCAGCCCGGGATTCAAAGGAATCAGGTGTGACCAAG
Seq A exon
GTAACACAGGTGGCAGAGTGCCTGGACAGGGGTGGAAAGGGCCCAACTACTGGTGGCTTTGGAGAGTTCTAACCACACCAGTTGAGTTTTATGACACTACCTTTTATTCATAGTTGGAAGCCTCTCTTCACAGCTTCTCTGTGCATATCCTCTGGTAGTTCCCCCAGGAGCGAGGCAAGAATGAGCCACAAGCTAGTTCTGGGAAAGGACATCAGGCTAAGTTGGAAACACCTGCTTAGTTTGACTTCTGCCACATCCCGATAGGTGTCTCTGGGCGTGTCATTTTCTTTGCTGGGTCTTTTACTCCACATTCAATTAAGTAAATGTTAACCTTTGTCCAAAGGGTTCAACTTGACTCTAGTTCTATGCCTAGGCGACATTGCATGGTACAGCTGAGAGGGATCAAATCCAGTCAACTCAAGAAGGGTCTCAGCACAGTGCCTGGCACATGTGAGCACTCTGTAAATGATGGCTTCGTGCATGGGAAATCCCTTTGTTTTGTAGACATGGGAGGCTATTGTTAGTTCTAGAAGATGGGAACAGAAAATAGGCTCTCAATTACCCACATCAGACACATGACTCTGCAATGTGAAGCCATGCAGCTGAGGCAAATGCAACATTTGCCTTTCTAGTAGTGACAACCTGCTTTCTTCAGCCAGGAGCAGAGAACCAAGGGGAGGTCTAGATGCTGCACAGCTAGGGAACAGACCTTCGCCAGGGCAGTCACAGAGGGACCTAGCCACACATGTATAATCAAGAGGTGTCTTAGGGCAGTAATAGTCACAGAGCTTCCAGCTGTCAAGGCCAGTCTGTCATTTGGTGTTCATGACTCCCCAAGGAAGCTGGAGTTGCTGTCCCCATAAACCTTCTAAAGCCACATGGCTGGTAGAGCAGGAAGAGTAGCTGAAACCAGTTTTTCCTGACTGAATTACTGGCTGGGTGAACTTAGACAAGTTCCCTAACCTTGGTGACTCAGTTTATTTATTTACTTTCCAACCAAGTAGAAATGGTAAAAATAATTAGTTCCCTTTTTGTGGGCCTGCCATGGTAAAGCAGGTAACTTGAACTCATGTGTGGACCTGAGTAAAATATTCAGTAAGCTTTTATTTTTGCTTCACACCTAAATCACATTTTCTTTTTGTAACCCTGAGACTCAATCACTTTCTGGCATTAGAAGAAACGGAGGCACTGTGAGGGTTGGGATTAGGCAGGGCTGAAGGGGCAAAGGCAGGATGGAAATATTGGGGTCAGGCCCACCTCTGGCTCAGGCCTCCCTCTGCCCACAG
Seq C2 exon
CTGCCCTCATGATGGATGAGCTGAATCCATACACCAAGATCAGTCCAGCTCTGGGAGCAGAGCGGCACTCGGTGGGTGCTGTCACAGGCATCATCCTCCTGTTGTTCCTGGTTGTGGTGTTGCTGGGCCTGTTTGCCTGGCGACGGAGGCGGCAGAAAGAGAAAGGCCGTGACCTGGCTCCTCGAGTCTCCTACACCCCAGCCATGAGGATGACCAGCACAGACTACTCTCTCTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000010634:ENSRNOT00000037941:18
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.050
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=PD(30.2=29.5),PF0005319=Laminin_EGF=PU(82.9=65.9)
A:
NA
C2:
PF0005319=Laminin_EGF=PD(14.3=6.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCCCCTGGAACCTTTGGATA
R:
AGTCTGTGCTGGTCATCCTCA
Band lengths:
350-1636
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]