RnoINT0095753 @ rn6
Intron Retention
Gene
ENSRNOG00000019846 | Muc5b
Description
mucin 5B, oligomeric mucus/gel-forming [Source:RGD Symbol;Acc:1561983]
Coordinates
chr1:214782529-214782916:+
Coord C1 exon
chr1:214782529-214782619
Coord A exon
chr1:214782620-214782809
Coord C2 exon
chr1:214782810-214782916
Length
190 bp
Sequences
Splice sites
5' ss Seq
ACAGTGAGT
5' ss Score
8.34
3' ss Seq
CTGAACTTTCTCTCCTGCAGACA
3' ss Score
11.24
Exon sequences
Seq C1 exon
CTGGAACTGGACTCCAAATACACCAACCAGACGTGTGGGCTGTGCGGTGACTTCAACGGGCTCCCAGCTGTCAATGAGTTCTACGCCCACA
Seq A exon
GTGAGTGTCACTGAGTCTTCGGGCTAGATGGTCCTAGGGGATAGAAAATCTAGGAAGTCCAGGAGGGATTGCAGACATGGAGGATGGAAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTCCAGGTAAAACCCCCGATAATCAGACCTCTTGACTGAACTTTCTCTCCTGCAG
Seq C2 exon
ACACTAGGCTGACCCCTGTCCAGTTTGGAAACCTGCAGAAGCTGGATGGTCCCACAGAACAGTGCCAGGATGTCCTACTGTCACCTGCCAACAACTGCACAGATACG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000019846:ENSRNOT00000028967:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0009420=VWD=FE(20.3=100)
A:
NA
C2:
PF0009420=VWD=PD(0.7=2.8),PF087426=C8=PU(4.2=8.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGAACTGGACTCCAAATACACC
R:
ATCTGTGCAGTTGTTGGCAGG
Band lengths:
194-384
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]