RnoINT0096495 @ rn6
Intron Retention
Gene
ENSRNOG00000046276 | Myh3
Description
myosin heavy chain 3 [Source:RGD Symbol;Acc:3138]
Coordinates
chr10:53643995-53644339:+
Coord C1 exon
chr10:53643995-53644099
Coord A exon
chr10:53644100-53644243
Coord C2 exon
chr10:53644244-53644339
Length
144 bp
Sequences
Splice sites
5' ss Seq
CAGGTAGGT
5' ss Score
10.28
3' ss Seq
ATCCCTTCATTCATTTGCAGAGT
3' ss Score
9.32
Exon sequences
Seq C1 exon
ATCAGAGAGCTAGAGTTTGAGCTGGAAGGGGAACAGAAGAGGAACACAGAGTCTGTGAAGGGCCTGAGGAAGTATGAGCGGCGTGTCAAGGAGCTCACCTACCAG
Seq A exon
GTAGGTGCAAAGGTCACCTAAGACAAGTTTTCTCTCCAACGTACAAATACTACTCAAAGCTTGGAATTGGACACTTGTGCACCCCTGGACTGGCTGGGCCCTGGTACAAGTGCCCCCCCCCCCCATCCCTTCATTCATTTGCAG
Seq C2 exon
AGTGAGGAGGACAGGAAGAATGTGCTGAGATTGCAGGATCTGGTGGATAAACTTCAAGTGAAAGTCAAGTCCTACAAGAGGCAGGCTGAGGAGGCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000046276:ENSRNOT00000004147:39
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.400 A=NA C2=0.156
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(4.0=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(3.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGAGAGCTAGAGTTTGAGCTGGA
R:
TCCTCAGCCTGCCTCTTGTAG
Band lengths:
194-338
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]