RnoINT0108923 @ rn6
Intron Retention
Gene
ENSRNOG00000000065 | Pde6b
Description
phosphodiesterase 6B [Source:RGD Symbol;Acc:1311039]
Coordinates
chr14:2331693-2332044:-
Coord C1 exon
chr14:2331981-2332044
Coord A exon
chr14:2331768-2331980
Coord C2 exon
chr14:2331693-2331767
Length
213 bp
Sequences
Splice sites
5' ss Seq
AAGGTCAGG
5' ss Score
6.23
3' ss Seq
TAACTTTTGTGTGTGCTCAGGTT
3' ss Score
9.04
Exon sequences
Seq C1 exon
GGCCATGATGATGACTGCATGTGACCTGTCTGCTATCACCAAGCCCTGGGAAGTCCAGAGCAAG
Seq A exon
GTCAGGACTGATGACCATCTGGACCCAGAGTAGCCCTTCACAGGCCGTCCTGGGTCTGTACCTAGGAAGCTACAGGGAAATGGTGTTGGCTTTTGAAGTAATGCCTGGGTACAGCAATGAGAGGCACCATACGAACACTGCCTGCAGAGCCTCGAGGGGAGGGCCGGCTGCTCCTGAATACAGAGCCCCACTATAACTTTTGTGTGTGCTCAG
Seq C2 exon
GTTGCTCTTCTGGTGGCTGCTGAGTTCTGGGAACAGGGGGACCTGGAGAGGACTGTCCTGGACCAACAGCCCATT
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000000065:ENSRNOT00000000077:18
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.040
Domain overlap (PFAM):
C1:
PF0023314=PDEase_I=FE(8.4=100)
A:
NA
C2:
PF0023314=PDEase_I=FE(9.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCCATGATGATGACTGCATG
R:
GCTGTTGGTCCAGGACAGTC
Band lengths:
134-347
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]