RnoINT0133054 @ rn6
Intron Retention
Gene
ENSRNOG00000004033 | Sema6a
Description
semaphorin 6A [Source:RGD Symbol;Acc:1305493]
Coordinates
chr18:41374386-41375280:-
Coord C1 exon
chr18:41375161-41375280
Coord A exon
chr18:41374475-41375160
Coord C2 exon
chr18:41374386-41374474
Length
686 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAGC
5' ss Score
9.6
3' ss Seq
AAGTGCCCTTTTGTCTACAGAGC
3' ss Score
7.25
Exon sequences
Seq C1 exon
ATGGGAAACTGTACTCGGCCACAGTGACTGACTTTCTGGCCATTGATGCGGTCATTTACAGGAGCCTTGGAGACAGTCCTACCCTCAGGACTGTCAAGCATGATTCAAAGTGGTTGAAAG
Seq A exon
GTGAGCAGTTAAAAGGAGAGAGGGAGGAGGGGATGCATACCTCATAAGAGATGTAGTCTGGAAGGACCAGACCGTGGATTCATTTTAGTAATGGTTTCTGACAGACAAAACCATGTAAATTGCATCTTTTGCTCTCAACTGATTTATTTACCTCCCTTTTCCCCTTCCCACGAGCTTCTGGAGTACTAACAAAACTACCAGTGCTGAGACAATAGACTAGGCCACATTCATCTTTCCCTAACTCAAATTCATTTCAGAGTCCCCAATGAAAAGAGCTGAACAGAACATTCATGAAAAGGAACGGGGGGGGGGGGGGCTGGAGGTGGTGGGGAACAGGAAAGGGGCTATTCAGATCCCTGAGTGAGTCAGCGTGATGGAGCAATGTGCAAAATCTCCTCTAGGCGATGTGGACATTCCATTGTGGAAAATAAAATGACAGAGATTTGCAAAACGTACTTATTCTTCACTCCCTGTTGCAAAGCTTGTGAAAGATGTAATTTGTAGGCTCAAGTGCCTTCTGAAATCACTAGCGCTTGCACTTCTGAAGTCATTAGAAATGGTTAGTGTTAACCACATGTCTAATAGCCTTACACTTCCAATTAATAACACGTCTTTGGCTACACTTGATTAAAATTCCCCTAACACTCTGTGACATGTCTCCAATTAAAGTGCCCTTTTGTCTACAG
Seq C2 exon
AGCCGTACTTTGTCCAAGCAGTGGATTACGGAGACTATATCTACTTCTTCTTCAGAGAAATTGCAGTAGAGTACAACACTATGGGGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000004033:ENSRNOT00000005446:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(9.2=100)
A:
NA
C2:
PF0140314=Sema=FE(6.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGGGAAACTGTACTCGGCCA
R:
CCCCATAGTGTTGTACTCTACTGC
Band lengths:
206-892
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]