BtaEX0005297 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000005676 | BAT8
Description
Bos taurus euchromatic histone-lysine N-methyltransferase 2 (EHMT2), mRNA. [Source:RefSeq mRNA;Acc:NM_001206263]
Coordinates
chr23:27282871-27283608:+
Coord C1 exon
chr23:27282871-27282985
Coord A exon
chr23:27283132-27283233
Coord C2 exon
chr23:27283460-27283608
Length
102 bp
Sequences
Splice sites
3' ss Seq
TCTCTCTGTCTCTGTGTCAGGAG
3' ss Score
8.74
5' ss Seq
CTGGTAATT
5' ss Score
5.99
Exon sequences
Seq C1 exon
AGTGGTTCCAGCGGCCGCCGCAAAGCCAAGAAGAAATGGCGGAAGGACAGCCCATGGGTGAAGCCATCACGGAAACGGCGGAAACGAGAGCCTCCGAGGGCCAAGGAGCCACGAG
Seq A exon
GAGTGAATGGTGTGGGCTCCTCAGGCCCCAGTGAGTACATGGAGGTCCCTCTGGGGTCTCTGGAGCTGCCCAGCGAGGGGACCCTCTCTCCCAACCACGCTG
Seq C2 exon
GGGTGTCCAATGACACATCTTCGCTGGAGACAGAGCGCGGGTTTGAGGAGCTGCCCCTCTGCAGCTGCCGCATGGAGGCCCCCAAGATAGACCGCATCAGCGAGAGAGCAGGGCACAAGTGCATGGCCACAGAGAGCGTGGACGGAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000005676_CASSETTE3
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.744 A=1.000 C2=0.200
Domain overlap (PFAM):
C1:
PF0334410=Daxx=FE(15.4=100)
A:
PF0334410=Daxx=FE(13.8=100)
C2:
PF0334410=Daxx=FE(19.9=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCCGCAAAGCCAAGAAGAAAT
R:
CTCTCCGTCCACGCTCTCTG
Band lengths:
248-350
Functional annotations
There are 2 annotated functions for this event
PMID: 11707778
The sub-cellular distribution of the NG36/G9a-T7 and G9a-T7 proteins was found to be quite distinct. Whereas the G9a-T7 protein was observed in both the cytoplasm and the nucleus, the NG36/G9a-T7 protein was extensively concentrated within the nucleus. Also, the G9a-T7 protein frequently appeared marginalized at the nuclear periphery, while the NG36/G9a-T7 protein was generally found throughout the nucleoplasm.
PMID: 26997278
Although E10 inclusion greatly stimulates overall H3K9me2 levels, it does not affect G9a catalytic activity. Instead, E10 increases G9a nuclear localization. The authors show that the G9a E10(+) isoform is necessary for neuron differentiation and regulates the alternative splicing pattern of its own pre-mRNA, enhancing E10 inclusion.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development