MmuEX0016483 @ mm9
Exon Skipping
Gene
ENSMUSG00000013787 | Ehmt2
Description
euchromatic histone lysine N-methyltransferase 2 [Source:MGI Symbol;Acc:MGI:2148922]
Coordinates
chr17:35042287-35043003:+
Coord C1 exon
chr17:35042287-35042401
Coord A exon
chr17:35042555-35042656
Coord C2 exon
chr17:35042855-35043003
Length
102 bp
Sequences
Splice sites
3' ss Seq
TCTCTCTGTCTCTGTGTCAGGAG
3' ss Score
8.74
5' ss Seq
CTGGTAATT
5' ss Score
5.99
Exon sequences
Seq C1 exon
AGCGGTTCTAGTGGCCGGCGCAAGGCCAAGAAGAAATGGCGGAAAGACAGCCCGTGGGTGAAGCCATCTAGAAAACGGCGGAAACGAGAGCCTCCGAGGGCCAAGGAGCCAAGAG
Seq A exon
GAGTGAATGGTGTGGGTTCCTCAGGGCCCAGTGAGTACATGGAGGTTCCTCTGGGGTCCCTGGAGCTGCCCAGCGAGGGGACCCTCTCCCCCAACCACGCTG
Seq C2 exon
GGGTCTCCAATGACACGTCTTCACTGGAGACAGAACGCGGGTTTGAGGAGCTGCCCCTCTGCAGCTGCCGCATGGAGGCTCCCAAGATTGACCGCATCAGCGAGAGAGCAGGGCACAAGTGCATGGCCACAGAGAGTGTGGATGGAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000013787_CASSETTE2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.731 A=1.000 C2=0.233
Domain overlap (PFAM):
C1:
PF041477=Nop14=FE(27.7=100),PF0334410=Daxx=FE(18.8=100)
A:
PF041477=Nop14=PD(1.5=5.7),PF0334410=Daxx=FE(16.8=100)
C2:
PF041477=Nop14=PD(6.9=20.0),PF0334410=Daxx=PD(29.7=94.0)
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCGCAAGGCCAAGAAGAAATG
R:
CTCTCCATCCACACTCTCTGT
Band lengths:
247-349
Functional annotations
There are 2 annotated functions for this event
PMID: 26997278
Although E10 inclusion greatly stimulates overall H3K9me2 levels, it does not affect G9a catalytic activity. Instead, E10 increases G9a nuclear localization. The authors show that the G9a E10(+) isoform is necessary for neuron differentiation and regulates the alternative splicing pattern of its own pre-mRNA, enhancing E10 inclusion.
PMID: 11707778
The sub-cellular distribution of the NG36/G9a-T7 and G9a-T7 proteins was found to be quite distinct. Whereas the G9a-T7 protein was observed in both the cytoplasm and the nucleus, the NG36/G9a-T7 protein was extensively concentrated within the nucleus. Also, the G9a-T7 protein frequently appeared marginalized at the nuclear periphery, while the NG36/G9a-T7 protein was generally found throughout the nucleoplasm.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: