BtaEX0006814 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000002297 | CACNB4
Description
calcium channel, voltage-dependent, beta 4 subunit [Source:HGNC Symbol;Acc:HGNC:1404]
Coordinates
chr2:44186533-44401136:+
Coord C1 exon
chr2:44186533-44186616
Coord A exon
chr2:44316598-44316757
Coord C2 exon
chr2:44401017-44401136
Length
160 bp
Sequences
Splice sites
3' ss Seq
AGTTTATTTGTTTTTATAAGGAA
3' ss Score
5.4
5' ss Seq
GCTGTAAGT
5' ss Score
8.56
Exon sequences
Seq C1 exon
GTGGCCCGAGGCACCACGACCCGGAGGAGCAGGTTGAAAAGATCCGATGGCAGCACCACTTCGACCAGCTTCATCCTCAGACAG
Seq A exon
GAAGAATAATGTGATGTGTTACCCGAGATGGTGTGAACAAAGCTTCAAGCCAATGGAGAAGACTAGGCCTAAATAGCTGACCCGGTGAAGTGAAAAATAAAGCCACACCTGGAGAGCCACATCCTCTGCAGTCTTTCTCAATGCCAATGCATTTATGGCT
Seq C2 exon
GGTTCAGCGGACTCCTACACAAGCAGGCCGTCTGACTCCGATGTCTCTTTGGAAGAGGATCGGGAAGCGATTCGGCAGGAGAGGGAGCAGCAGGCGGCCATCCAGCTTGAGAGAGCAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000002297_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.982 A=1.000 C2=0.875
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF120523=VGCC_beta4Aa_N=PU(92.9=97.5)
Main Inclusion Isoform:
ENSBTAT00000002971fB1287
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGGAGGAGCAGGTTGAAAAGA
R:
TTGCTCTCTCAAGCTGGATGG
Band lengths:
181-341
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development