BtaEX0013028 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000001552 | EPC1
Description
enhancer of polycomb homolog 1 (Drosophila) [Source:HGNC Symbol;Acc:HGNC:19876]
Coordinates
chr13:33507050-33514923:+
Coord C1 exon
chr13:33507050-33507402
Coord A exon
chr13:33514050-33514166
Coord C2 exon
chr13:33514805-33514923
Length
117 bp
Sequences
Splice sites
3' ss Seq
TTTTTGTTTGTTTTTTTTAGAAC
3' ss Score
10.98
5' ss Seq
CAGGTGGGT
5' ss Score
8.56
Exon sequences
Seq C1 exon
GGTCTTACTGGACAGAGCGCACTCAGACTACGACAGCGTGTTTCGCCATCTGGATCTGGACATGCTTTCCTCACCACAACATTCTCCAGTCAATCAGTCTGCCAATACCTCAGAAACAAATACCTCGGACAAATCTTTCTCTAAAGACCTCAGTCAGATACTCGTCAATATCAAATCATGTAGATGGCGGCATTTTAGGCCTCGGACACCATCCCTACATGACAGTGACAATGATGAGCTCTCCTGTCGAAAACTGTATAGGAGTATAAATCGAACAGGCACAGCACAACCCGGGACCCAGACATGCAGTACCTCTACGCAAAGCAAAAGCAGCAGTGGTTCAGCACACTTTG
Seq A exon
AACTGAGCTCTCCATAGACAAACATAAGTAGCATAACACAGTGTCTTTCCTCAGACATCATTCTGTACAGTAAGCCAACATAAACAACTCCGTTCTTATTGACTGGATTTTTTCCAG
Seq C2 exon
CATTTACAGCCGAACAATACCAGCAACATCAACAGCAGCTGGCACTCATGCAGAAACAGCAGCTTGCACAAATTCAGCAACAGCAAGCAACTAGTAATTCCTCCACCACCGTGCCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000001552_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.517 A=1.000 C2=0.600
Domain overlap (PFAM):
C1:
PF067527=E_Pc_C=PU(0.1=0.0)
A:
NO
C2:
PF067527=E_Pc_C=PU(14.9=97.5)
Main Inclusion Isoform:
ENSBTAT00000002038fB2474
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCATTTTAGGCCTCGGACACC
R:
TGCTGAATTTGTGCAAGCTGC
Band lengths:
243-360
Functional annotations
There are 2 annotated functions for this event
PMID: 31911676
CRISPR targeting of the poised exons showed a modest fitness advantage in cell culture. Tumors derived from engraftment of polyclonal EPC1 poison exon targeted PC9-Cas9 cells were significantly larger and exhibited increased Ki-67 staining relative to control tumors.
PMID: 31911676
[CRISPR screen]. Conserved poison exon with pro-oncogenic impact when depleted: HeLa No Change at 14 days (FC=1.187, FDR=0.002), PC9 No Change at 14 days (FC=1.144, FDR=0.102), Late Xenograft Enriched (FC=2.344, FDR=0.000).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development