RnoEX0032465 @ rn6
Exon Skipping
Gene
ENSRNOG00000014834 | Epc1
Description
enhancer of polycomb homolog 1 [Source:RGD Symbol;Acc:2324280]
Coordinates
chr17:57309799-57316917:-
Coord C1 exon
chr17:57316565-57316917
Coord A exon
chr17:57310482-57310593
Coord C2 exon
chr17:57309799-57309917
Length
112 bp
Sequences
Splice sites
3' ss Seq
TTTTTGTTTGTCTTTTTTAGAAC
3' ss Score
12.32
5' ss Seq
CAGGTGGGT
5' ss Score
8.56
Exon sequences
Seq C1 exon
GGTCTTGCTGGACAGAGCGCATTCAGACTATGACAGTATGTTTCACCACCTGGATCTGGACATGCTTTCCTCACCACAACCTTCTCCAGTCAATCAGTTTGCCAATACCTCAGAAACCAATACCTCGGACAGACCTTCCTCTAAAGACCTCAGTCAGATACTAGTCGATATCAAATCATGTAGATGGCGGCACTTTAGGCCCCGGACACCATCCCTTCCTGACAGTGACAGTGGTGAGCTCTCCAGTAGAAAGTTACACAGAAGTATAAGTCGAGCAGGACCAGCGCAGCCTGGGGCCCACACGTGCAGCGCCTCCACACAGAGCAGAAGTAGCAGTGGCTCGACACACTGTG
Seq A exon
AACTGAGCTCTCCATAGACACACATAAGTAGCATAACACAGTGTCTGTCCTCAGGCTTCACTCTGTACAGTAATCAGCATTACTCCGTTCTTATTGACTGGATTTTGTCCAG
Seq C2 exon
CATTTACAGCCGAGCAGTACCAGCAGCACCAGCAGCAACTGGCACTCATGCAGCAGCAGCAGCTTGCACAAACTCAGCAGCAGCAAGCAAATAGCAGTTCCTCCACCGCCGCTCCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000014834_MULTIEX1-2/4=1-3
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.697 A=1.000 C2=0.850
Domain overlap (PFAM):
C1:
PF067527=E_Pc_C=PU(0.1=0.0)
A:
NO
C2:
PF067527=E_Pc_C=PU(16.8=97.5)
Main Inclusion Isoform:
ENSRNOT00000019968fB4886
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACACCATCCCTTCCTGACAGT
R:
GCTATTTGCTTGCTGCTGCTG
Band lengths:
243-355
Functional annotations
There are 2 annotated functions for this event
PMID: 31911676
CRISPR targeting of the poised exons showed a modest fitness advantage in cell culture. Tumors derived from engraftment of polyclonal EPC1 poison exon targeted PC9-Cas9 cells were significantly larger and exhibited increased Ki-67 staining relative to control tumors.
PMID: 31911676
[CRISPR screen]. Conserved poison exon with pro-oncogenic impact when depleted: HeLa No Change at 14 days (FC=1.187, FDR=0.002), PC9 No Change at 14 days (FC=1.144, FDR=0.102), Late Xenograft Enriched (FC=2.344, FDR=0.000).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]