Special

BtaEX0021575 @ bosTau6

Exon Skipping

Gene
Description
Bos taurus muscleblind-like 2 (Drosophila) (MBNL2), mRNA. [Source:RefSeq mRNA;Acc:NM_001099710]
Coordinates
chr12:78409734-78445751:+
Coord C1 exon
chr12:78409734-78409887
Coord A exon
chr12:78418797-78418891
Coord C2 exon
chr12:78444069-78445751
Length
95 bp
Sequences
Splice sites
3' ss Seq
CATGGTTCCCCTCCTGAAAGTAC
3' ss Score
3.97
5' ss Seq
CAGGTTTGC
5' ss Score
4.86
Exon sequences
Seq C1 exon
GCCTTCCCTCCTGGTGCTCTTCATCCTTTACCAAAGAGACAAGCACTTGAAAAAAGCAACGGTGCGAGCACCGTGTTTAACCCCAGCGTCTTGCAGTACCAGCAGGCTCTCACCAGTGCGCAGCTGCAGCAGCACACCGCGTTCATCCCGACAG
Seq A exon
TACCCATGATGCACAGCGCTACGTCAGCCACTGTCTCTGCAGCAACAACTCCTGCAACAAGTGTCCCCTTCGCAGCAACAGCCACAGCCAATCAG
Seq C2 exon
ATAATTCTGAAATAATCAGCAGGAACGGAATGGAATGCCAAGAATCTGCATTGAGAATAACTAAACATTGTTACTGTACATACTATCCTGTTCCCTCCTCAATAGAATTGCCACAAACTGCATGCTAAATAAAGATTTAGTTCTTCTGGACAGACCACAACTCTAAGAAGCTAGTGCTGCTATATCATATATGAGTATTAAATATGGTATGCTTAGTATATTCCAACCTAAGATAGTTAACTACCTGAGACCAGCTGTGATGTTAAAGGACATAAAGGATAAAGTTTACTTTTAAAGGGTTTCTAAACATAGTTTCTGTCCTAGGAATATTGTCTTCTCTCCATAACTATAGCTGATGCAGAAAGTCCAACCAATTTATTCATTTCGACTCAGAATATTTCAAAGTTAGCAATAAACAATTAGCATTAGTTAAAAAAAAAAGAAAAAGAAAGAAAGAAAAAAAACCTATTCCAGGGGCAGGTTCAATTCTAACTTCAATT
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000018313_MULTIEX2-2/3=C1-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact

In the CDS, with uncertain impact

No structure available
Features
Disorder rate (disopred):
  C1=0.159 A=0.969 C2=0.300
Domain overlap (PFAM):

C1:
NO
A:
NO
C2:
NO


Main Inclusion Isoform:
ENSBTAT00000030532fB4136


Main Skipping Isoform:


Other Inclusion Isoforms:
NA


Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
ALTERNATIVE
Zebrafish
(danRer10)
ALTERNATIVE
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTCCTGGTGCTCTTCATCCT
R:
TCCATTCCGTTCCTGCTGATT
Band lengths:
182-277
Functional annotations
There are 1 annotated functions for this event
PMID: 27733504
The presence of alternative ex.54nt always significantly elevated MBNL1 and MBNL2 protein levels (_2.7 and _4.0-times, respectively). There were marginal changes of the GFP-MBNL level for constructs carrying ex.36nt (_1.3-times) and ex.95nt (_0.8-times). There were no significant differences between the activities of the analyzed proteins for the majority (67%) of AS events. However, these exons might have either a positive or negative effect on some specific AS events (Figure 5A and C). Interestingly, exOFF events predominated in AS events with a negative effect of ex.36nt (95%; P = 0.002) and a positive effect of ex.95nt (90%; P = 0.010). All together, these data indicate that the presence of sequences encoded by these alternative exons can significantly modulate MBNL splicing activity, but this regulation depends strongly on the targeted RNA.


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Pre-implantation embryo development