BtaEX6009002 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000020984 | RAPGEF4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:HGNC Symbol;Acc:HGNC:16626]
Coordinates
chr2:23711292-23716588:-
Coord C1 exon
chr2:23716504-23716588
Coord A exon
chr2:23713742-23713802
Coord C2 exon
chr2:23711292-23711368
Length
61 bp
Sequences
Splice sites
3' ss Seq
TTTTTCTCTTTTTCTTCTAGGTG
3' ss Score
14.12
5' ss Seq
TGTGTAAGT
5' ss Score
7.65
Exon sequences
Seq C1 exon
ACCTGGACAGAGGACCATGGATGACCTAGAAATTATCTATGAAGAACTCCTTCACATTAAAGCCTTATCCCATCTTTCTACCACA
Seq A exon
GTGAAACGGGAGTTGGCAGCGGTTCTCATTTTTGAGTCTCATGCCAAAGGAGGGACTGTGT
Seq C2 exon
TATTTAACCAAGGGGAAGAAGGCACCTCCTGGTACATTATTCTAAAAGGATCAGTGAATGTAGTCATTTATGGCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000020984-'25-23,'25-22,27-23=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0002724=cNMP_binding=PU(10.3=42.9)
C2:
PF0002724=cNMP_binding=FE(28.7=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTGGACAGAGGACCATGGAT
R:
TCCTTTTAGAATAATGTACCAGGAGG
Band lengths:
134-195
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]