BtaEX6026660 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000015539 | RAPGEF5
Description
Rap guanine nucleotide exchange factor (GEF) 5 [Source:HGNC Symbol;Acc:HGNC:16862]
Coordinates
chr4:31034078-31042545:-
Coord C1 exon
chr4:31042423-31042545
Coord A exon
chr4:31040227-31040405
Coord C2 exon
chr4:31034078-31034168
Length
179 bp
Sequences
Splice sites
3' ss Seq
TTGAATACATTGTCTTTCAGCAA
3' ss Score
6.7
5' ss Seq
TCAGTGAGT
5' ss Score
7.68
Exon sequences
Seq C1 exon
AATCCGTTTGCAGAAAATGAGGAATCACAGCAAAGGTCGATCAGGATTTTGGGAATGAACACTTGGGATCTTGCTCTGGAATTAATGAATTTTGATTGGAGCCTTTTCAGTTCAATTCACGAG
Seq A exon
CAAGAGCTGATCTACTTCACGTTCAGCAGACAGGGAAGCGGGGAGCACACGGCGAACCTGAGCCTGCTGCTCCAGAGATGCAATGAGGTCCAGCTCTGGGTGGCCACGGAGATTCTGCTCTGCAGCCCACTGGGCAAGCGAGTGCAGCTGGTGAAAAAATTCATCAAAATTGCCGCTCA
Seq C2 exon
CTGCAAAGCCCAGAGAAACCTGAACTCCTTCTTTGCTATCGTGATGGGTCTCAACACTGCTTCTGTGAGCCGGCTATCTCAGACCTGGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000015539-'24-36,'24-34,25-36=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.024 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0061714=RasGEF=PU(13.0=58.5)
A:
PF0061714=RasGEF=FE(31.9=100)
C2:
PF0061714=RasGEF=FE(16.2=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCGTTTGCAGAAAATGAGGA
R:
CTCCCAGGTCTGAGATAGCCG
Band lengths:
212-391
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]