DreEX6040383 @ danRer10
Exon Skipping
Gene
ENSDARG00000074307 | rapgef5b
Description
Rap guanine nucleotide exchange factor (GEF) 5b [Source:ZFIN;Acc:ZDB-GENE-061215-2]
Coordinates
chr16:19335964-19339487:+
Coord C1 exon
chr16:19335964-19336080
Coord A exon
chr16:19337901-19338079
Coord C2 exon
chr16:19339397-19339487
Length
179 bp
Sequences
Splice sites
3' ss Seq
ATATTGTCCCCACCCTGCAGCAA
3' ss Score
8.19
5' ss Seq
ACAGTAAGT
5' ss Score
9.49
Exon sequences
Seq C1 exon
AGTCCATTCACTGATAATGGGCAGCAGTGTTCAGGAAAGATGCTGAGCATGAACACATGGGATGTGTCAGTGTCGCTCACTAACTGCGACTGGAGTCTCTTCAGTAACATCCATGAG
Seq A exon
CAAGAGTTGGTGTATTTTACTCTGAGTCGTGACTCAAGTTCTGGCCACACGGTGGCGCTGGAGCAGCTGTTGCAACGCTGCAATGAGACTCAGCAGTGGGTGATGACTGAAGTCCTTCTGTGTTCAACCCTCTGTAAACGAGTGCAGCTCTTCAAGAAGTTCATCAAGATCGCAGCACA
Seq C2 exon
CTGTAAAGCACAGAGGAACCTGAACTCATTATTTGCGATTATTCTGGGACTGAACAGTCCTGCTGTCAGTCGACTTACTCAGACATGGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000074307-'26-31,'26-30,28-31=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0061714=RasGEF=PU(13.0=61.5)
A:
PF0061714=RasGEF=FE(31.9=100)
C2:
PF0061714=RasGEF=FE(16.2=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCATTCACTGATAATGGGCAGC
R:
TCCCATGTCTGAGTAAGTCGACT
Band lengths:
205-384
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]