BtaEX6063088 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000032544 | SPAG1
Description
sperm associated antigen 1 [Source:HGNC Symbol;Acc:HGNC:11212]
Coordinates
chr14:66334533-66370011:-
Coord C1 exon
chr14:66369852-66370011
Coord A exon
chr14:66346003-66346176
Coord C2 exon
chr14:66334533-66334699
Length
174 bp
Sequences
Splice sites
3' ss Seq
AGGAGCGCTGCCTCTCCCAGGGG
3' ss Score
4.65
5' ss Seq
GCCGGGAGC
5' ss Score
-4.16
Exon sequences
Seq C1 exon
AAAACCTTGTCAGAGGTTGAAAGAGATCTGAAAAATTCTGAACCTGCATCTAAGACTCAAACCAAGGGGAAAAGGATGGTTATTCAGGAAGTAGAAAACTCAGAAGATGAAGATGAAAAGGACAGTGGAATAAAACATGAAGATGGCAGTGGAGATAAGA
Seq A exon
GGGCGCCGGAACTGCCCGGCTCCCGGCGAGGGGCGGGCCGGCTCCTCCCGGCTTCGCCCGGGACCCCGCCCCCGCGCCGGCACTACCGCGGCCTCGGCGGTAGCTGCTCCGGATGATGCAAACAGTCACTTCCCGCATCCACTCCCCACAGAGGCCGCGGAGTCGGCGGGAGCC
Seq C2 exon
GATAACAAAGATTTTGATCGCTCTGGATGGCCCAAACTGGCGGGAGAAGCTGCCACCCATACCTGCTGTGCCCACCTCCACACAAGTCCGGGCTTGGCGGCCTGCAGCAGAGACGCCCCCAGACCAAGTGGGAGATTCCTGTTGCCATCCCCAGCCGGGCATGCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000032544-'12-24,'12-17,16-24=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=1.000 C2=0.386
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACCAAGGGGAAAAGGATGGTT
R:
TGGCAACAGGAATCTCCCACT
Band lengths:
247-421
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]