BtaEX6085151 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000013645 | RFFL
Description
ring finger and FYVE-like domain containing E3 ubiquitin protein ligase [Source:HGNC Symbol;Acc:HGNC:24821]
Coordinates
chr19:15378376-15395190:+
Coord C1 exon
chr19:15378376-15378570
Coord A exon
chr19:15387846-15388235
Coord C2 exon
chr19:15395107-15395190
Length
390 bp
Sequences
Splice sites
3' ss Seq
TGCCTCTCGCTCTTAAGCAGCAG
3' ss Score
3.07
5' ss Seq
CAGGTACGG
5' ss Score
10.88
Exon sequences
Seq C1 exon
GCATCAGATCTTATCATGTGGGCAACCTGCTGCAACTGGTTCTGCCTGGATGGACAGCCTGAGGAGGCCTCACCGCCCCAGGGAGCCAGGACACAGGCCTATTCCAACCCTGGGTACAGCTCCTTCCCTTCTCCCACGGGCTCAGAACCAAGCTGCAAGGCGTGTGGGACTCACTTCGCAAGCATGGCCAGGAAG
Seq A exon
CAGACCTGCTTGGACTGTAAGAAGAACTTCTGTACCAGCTGTTCCAGCCAAGTGGGGGCCGGGCCCCGCCTCTGCCTTCTCTGCCAGCGCTTCCGTGCCACGGACTTCCGGCGGGAGGAGCTCATGAAGATGAAGGTGAAGGACCTGAGGGACTATCTCAGCCTCCACGACATCTCTACCGACATGTGCCGGGAGAAGGAGGAGCTGGTGTCCCTGGTGCTCGGCCAGCAGCCCGTCATCTCCCAGGAGGGCAGGACTCAGGCGCCCCACTTGGGCCCCGACTTCCCGGAGCAGCAGGCCTTCCTCGCCCGGCCCCACACCAGCACCGTGCCGCCTACCTCACCCGGCCTCCAGTCTGCACCCCCCGGCCAGGCGCAGGGCCGGCAGCAG
Seq C2 exon
GCCAATGGCCATGTGTCTCAGGACCAAGAGGAGCCCATCTACCTGGAGCGCACAGCCAGAGCCCCTGCTGAGGACGAGACCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000013645-'3-6,'3-4,5-6=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.138 A=0.431 C2=1.000
Domain overlap (PFAM):
C1:
PF0231811=FYVE_2=PU(31.3=32.3)
A:
PF0231811=FYVE_2=PD(65.7=33.8),PF102084=Armet=WD(100=50.8)
C2:
PF102084=Armet=PU(12.3=35.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCATCAGATCTTATCATGTGGGCA
R:
CTGGGTCTCGTCCTCAGCAG
Band lengths:
279-669
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]