BtaEX6107577 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000000179 | RPUSD1
Description
Bos taurus RNA pseudouridylate synthase domain containing 1 (RPUSD1), mRNA. [Source:RefSeq mRNA;Acc:NM_001075874]
Coordinates
chr25:666948-667567:-
Coord C1 exon
chr25:667381-667567
Coord A exon
chr25:667182-667305
Coord C2 exon
chr25:666948-667050
Length
124 bp
Sequences
Splice sites
3' ss Seq
GAGCCTCTGCCCCATACTAGGTT
3' ss Score
6.1
5' ss Seq
CTGGTAAGG
5' ss Score
9.68
Exon sequences
Seq C1 exon
CCACCATGGAGCCAGGCAGCGTGGAGAATCTGTGTGTCGTGTACCGGAGCCATGACTTCCTGGTGGTGAACAAGCACTGGGACGTACGCATCGACAGCAAAGCCTGGCGGGAGACGCTGACCCTCCAGAAGCAGCTGCGGCACCGCTTCCCAGAGCTGGCCGACCCCGACACCTACTACGGGTTCAG
Seq A exon
GTTCTGCCACCAACTGGACTTCTCCACCAGCGGGGCACTCTGTGTGGCTTTGAACAAGGCAGCCGCAGGCAGTGCATACAGGTGCTTCAAGGACCGGCGAGTCACCAAGGCTTATCTGGCTCTG
Seq C2 exon
GTGCGGGGGCACGTCCAGGAGAGCCGAATGACCATCAGCTACGCCATCGGCAAGAACAGCACAGAAGGCCGGACCCACACCATGTGCATTGAAGGCACACAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000000179-'2-2,'2-1,3-2=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.114
Domain overlap (PFAM):
C1:
PF0084917=PseudoU_synth_2=PU(26.5=72.1)
A:
PF0084917=PseudoU_synth_2=FE(24.7=100)
C2:
PF0084917=PseudoU_synth_2=FE(20.5=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCTGTGTGTCGTGTACCGGA
R:
TGCCTTCAATGCACATGGTGT
Band lengths:
257-381
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]