DreEX6017687 @ danRer10
Exon Skipping
Gene
ENSDARG00000060192 | rpusd1
Description
RNA pseudouridylate synthase domain containing 1 [Source:ZFIN;Acc:ZDB-GENE-060929-444]
Coordinates
chr3:35412940-35415297:+
Coord C1 exon
chr3:35412940-35413133
Coord A exon
chr3:35414987-35415110
Coord C2 exon
chr3:35415195-35415297
Length
124 bp
Sequences
Splice sites
3' ss Seq
TGTGTTCTTATATTCTGCAGGTT
3' ss Score
10.26
5' ss Seq
CTGGTATTG
5' ss Score
3.14
Exon sequences
Seq C1 exon
GAGAAGACGAGCATGGAGCCTGCGAGCCTGGAAAACCTGTGTGTTTTGTATCAAAGTACAAACTATATAGTTGTCAACAAACACTGGGACATTCGAATTGACAGTAAAATGTGGTATGAGAAGCAGACTGTCCAGAGTCAGCTAAAGCATCGCTTTCCAGAGCTTGCAGATCCTGGCACTTACTACGGCTTCAG
Seq A exon
GTTTTGCCATCAGTTGGATTTCTCCACCAGTGGTGCTTTGTGTGTTGCTCTGAATAAGGCGGCAGCAGGTCAAGCGTATCGCTGTTTTAAAGACAGACGGGTTACCAAAGCTTATCTAGCACTG
Seq C2 exon
GTGCGTGGTACTGTGACTGAGGAGAATCTGAGCCTGGATTTCGCAATTGGCAAGAACACCACGGAGGGCAAAACCCATATGATGTGCACTGAAGGAACGGAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000060192-'1-5,'1-4,3-5=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.171
Domain overlap (PFAM):
C1:
PF0084917=PseudoU_synth_2=PU(26.5=72.1)
A:
PF0084917=PseudoU_synth_2=FE(24.7=100)
C2:
PF0084917=PseudoU_synth_2=FE(20.5=100)
Main Skipping Isoform:
ENSDART00000084549fB5331
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGCCTGGAAAACCTGTGTGT
R:
TTTTGCCCTCCGTGGTGTTC
Band lengths:
244-368
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]