RnoEX0077140 @ rn6
Exon Skipping
Gene
ENSRNOG00000023639 | Rpusd1
Description
RNA pseudouridylate synthase domain containing 1 [Source:RGD Symbol;Acc:1304934]
Coordinates
chr10:15099009-15100377:+
Coord C1 exon
chr10:15099009-15099242
Coord A exon
chr10:15099971-15100094
Coord C2 exon
chr10:15100275-15100377
Length
124 bp
Sequences
Splice sites
3' ss Seq
GTTCCTCTGCCCCTTCCCAGGTT
3' ss Score
9.56
5' ss Seq
CTGGTAAGC
5' ss Score
8.69
Exon sequences
Seq C1 exon
GACTAAGAAGGTGCGTGCGCAGACCATTCGGGAAGAGCTTCGCCTTCCTTCAGAGGGCGGAGCTCCATGCCGGGAATGCCATTGGCCAGTACGACCATCCATTAGCCAGGTGGAATGGGCAGGGTGAAGGCAGAGCTGCGCGGCAGCCAGGCCAAGACCTGGTTCCTTGTATAGGAAAGCTGGGTTGGATGCAGGGCTGAGACGTGACATCCTGGTCCTTTGGCCCTGTTGCAG
Seq A exon
GTTCTGCCACCAACTGGACTTCTCCACCAGCGGGGCGCTGTGCGTGGCCCTGAATAAAGCAGCTGCGGGCAGCGCTTATAAGTGCTTCAAGGAGCGACGAGTTACCAAAGCCTACCTGGCACTG
Seq C2 exon
GTCCGAGGCCATGTCCAGGAAAGCCAAGTGACGATCAACTATGCCATTGGCAGGAACAGCACAGAGGGGCGGACCCACACCATGTGCATTGAGGGCACTCATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000023639-'0-5,'0-4,2-5
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=NA A=0.000 C2=0.057
Domain overlap (PFAM):
C1:
NA
A:
PF0160011=Corona_S1=FE(30.4=100),PF0084917=PseudoU_synth_2=FE(24.7=100)
C2:
PF0160011=Corona_S1=PD(23.7=91.4),PF0084917=PseudoU_synth_2=FE(20.5=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACCATTCGGGAAGAGCTTCG
R:
ATCGTCACTTGGCTTTCCTGG
Band lengths:
248-372
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]