BtaINT0013082 @ bosTau6
Intron Retention
Gene
ENSBTAG00000017258 | ACSL3
Description
acyl-CoA synthetase long-chain family member 3 [Source:HGNC Symbol;Acc:HGNC:3570]
Coordinates
chr2:111867777-111868876:+
Coord C1 exon
chr2:111867777-111867886
Coord A exon
chr2:111867887-111868737
Coord C2 exon
chr2:111868738-111868876
Length
851 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAGA
5' ss Score
8.68
3' ss Seq
GGATCCTTGTGTGTTTCCAGGAC
3' ss Score
8.31
Exon sequences
Seq C1 exon
TTGTTACGCTGTATGCTACTCTAGGGGGTCCAGCAATTGTTCATGGATTAAATGAAACAGAGGTGACCAACATCATTACTAGTAAAGAACTCTTGCAGACAAAATTGAAG
Seq A exon
GTGAGAACTAGCTTTTCTACCTTGTGCTCATCTATTTTCAAGTCTATGTTCAATAAATTTTTAATTTTAAGCGTTATTTTTAAACTAGTAATTCACTTATTCTGAAGCATTGAGTACAAGTTTCAGAACTTTTTAAAGGAAAGTTAATGACTTTACGAAACTACAAGAGAAAGGAATTTTAGTTTCTGAACTCTATGAAAACAATAATATCTGATCCCCCCACCCCCCTTTTTTAAACAAGTTTATAAGAATATTCCCTTCCTCTCTCCCTATATCTTTATTTGCCTCTTAAAGCATGGGGAACATTTGGCTACCCTGTAGTTCAGGCAGTATGTGTTTATATGCCCATTTTTGCAAGGAATTAACTTTATTGATTAAAATTATACCAAAGAACAGGTATACACAGTAAATCTAATGTTATAAAGCAGTAACATATATTTCAAACCAATAGTTGTCCATCCGAAAAAAGGGGTAGTCATGCCAGTACACTTCACCCTTGCTTTTTTGAGGCAAGAAATTAACAATTTGAAACATGGTAAACTACTTTTCTTGTGCCCTGGATTATTTGCCACGCTCATTAATATAGAGTAATGAAGAATATGCTTCCCAGGTGGCGCAGTGGTAAAGCCTCCACCTGCCAGTGCAGGAGGTGCGGGTTCGATCCCTGCATGGGAAGATCCCCAGGAGTAGGAAATGACAAGCTACTCTAGTATTCTGGCTGGGATAATCCCATGGACAGAGGAGCCTGGTGGGCTGTAGTCCATGGGGTTGCAGAGTTGGACACGACTGAGCACGCACACACGTTCACTCATGTTCAGAGAAAACTGCAGTGGATCCTTGTGTGTTTCCAG
Seq C2 exon
GACATCGTTTCTCTGGTCCCTCGCCTGCGGCACATCATCACTGTAGATGGAAAGCCGCCGACCTGGTCTGAGTTCCCCAAGGGCGTCATCGTGCACACCATGGCGGCCGTGCGGGCTCTGGGAGCCAAGGCCAGCATGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000017258:ENSBTAT00000022939:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(7.6=100)
A:
NA
C2:
PF0050123=AMP-binding=FE(9.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTACGCTGTATGCTACTCTAGGGG
R:
CCATGCTGGCCTTGGCTC
Band lengths:
246-1097
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]