BtaINT0013167 @ bosTau6
Intron Retention
Gene
ENSBTAG00000006447 | ACSM3
Description
acyl-CoA synthetase medium-chain family member 3 [Source:HGNC Symbol;Acc:HGNC:10522]
Coordinates
chr25:18652909-18653240:+
Coord C1 exon
chr25:18652909-18653036
Coord A exon
chr25:18653037-18653140
Coord C2 exon
chr25:18653141-18653240
Length
104 bp
Sequences
Splice sites
5' ss Seq
CGGGTAATT
5' ss Score
6.79
3' ss Seq
CCTTATGTTTTATTTCCCAGTTA
3' ss Score
8.6
Exon sequences
Seq C1 exon
GATAATCCTACAAAAACAGCTTCAACTCTACGAGGCAATTTCTATATCACTGGGGACAGAGGCTATATGGATGAGGATGGATATTTCTGGTTTGTTTCAAGAGCAGATGATATCATATTATCTTCCGG
Seq A exon
GTAATTTTCATTTGTTGATATGTCTGTGTTGACTGCTTGTTAGTAGTCTGGAGTGAACTTTTTGCTCACCTGTATAAATTCTTGCCTTATGTTTTATTTCCCAG
Seq C2 exon
TTACCGAATTGGTCCGTTTGAGGTAGAAAGTGCCCTTATTGAACATCCTGCAGTTGCAGAGTCAGCTGTTGTCAGCAGTCCAGATCCCATCAGAGGAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000006447:ENSBTAT00000008455:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.023 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=PD(9.9=95.3)
A:
NA
C2:
PF131931=AMP-binding_C=PU(32.1=76.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TAATCCTACAAAAACAGCTTCAACT
R:
CTCTCCTCTGATGGGATCTGGA
Band lengths:
226-330
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]