RnoINT0012450 @ rn6
Intron Retention
Gene
ENSRNOG00000032246 | Acsm3
Description
acyl-CoA synthetase medium-chain family member 3 [Source:RGD Symbol;Acc:62086]
Coordinates
chr1:189534389-189534698:+
Coord C1 exon
chr1:189534389-189534516
Coord A exon
chr1:189534517-189534598
Coord C2 exon
chr1:189534599-189534698
Length
82 bp
Sequences
Splice sites
5' ss Seq
TGGGTAATT
5' ss Score
6.19
3' ss Seq
TTCCTGTCATATTTTCCTAGTTA
3' ss Score
6.36
Exon sequences
Seq C1 exon
GATAATCCTTCGAAAACAGCTTCAACTCTACGAGGGAATTTCTACATCACTGGGGACAGAGGCTATATGGATGAGGATGGCTATTTCTGGTTTGTTGCAAGATCAGACGATGTCATATTATCCTCTGG
Seq A exon
GTAATTTTCTATGCATGTCTATTTATAAGCATTCTGGAGTCAATCTAGCTCATCCTTATGAGTTCCTGTCATATTTTCCTAG
Seq C2 exon
TTACCGAATTGGACCATTTGAGGTAGAGAGTGCTCTCATAGAGCATCCTTCCATTGCAGAGTCAGCTGTTGTCAGCAGCCCTGACCCCATCAGAGGAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000032246:ENSRNOT00000020039:12
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.029
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=PD(9.9=95.3)
A:
NA
C2:
PF131931=AMP-binding_C=PU(32.1=76.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAGCTTCAACTCTACGAGGGA
R:
AGCTGACTCTGCAATGGAAGG
Band lengths:
180-262
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]