BtaINT0013202 @ bosTau6
Intron Retention
Gene
ENSBTAG00000037449 | ACSM5
Description
acyl-CoA synthetase medium-chain family member 5 [Source:HGNC Symbol;Acc:HGNC:26060]
Coordinates
chr25:18227011-18227331:+
Coord C1 exon
chr25:18227011-18227091
Coord A exon
chr25:18227092-18227229
Coord C2 exon
chr25:18227230-18227331
Length
138 bp
Sequences
Splice sites
5' ss Seq
CAGGTAGGC
5' ss Score
9.88
3' ss Seq
TCCTGTGATCTCTCTGCCAGGTT
3' ss Score
9.99
Exon sequences
Seq C1 exon
GTTGTAATCTGTGCCAATCCAAAAGGGATGAAAATCAAGTCAGGATCCATGGGGAAAGCGTCCCCACCATATGATGTGCAG
Seq A exon
GTAGGCAGCCTCTCCCAACTGTTGATAAGGCTTGAAGAAGAGAGCAAACATACATATCTGTATTAGCAATAGCAGACATCCGGCAGGGTGGCCTGGCTGTACAGACATCAAGACACCCTCCTGTGATCTCTCTGCCAG
Seq C2 exon
GTTGTAGATGAGGAGGGCAATATCTTACCTCCTGGAGAAGAAGGGAATGTTGCCATCCGTGTCAGACCCACCAGACCCTTCTGTTTCTTCACTTGCTATTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000037449:ENSBTAT00000054846:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.185 A=NA C2=0.353
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(6.3=100)
A:
NA
C2:
PF0050123=AMP-binding=FE(8.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTTGTAATCTGTGCCAATCCAA
R:
AGCAAGTGAAGAAACAGAAGGGT
Band lengths:
178-316
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]