Special

MmuINT0010295 @ mm10

Intron Retention

Gene
Description
acyl-CoA synthetase medium-chain family member 5 [Source:MGI Symbol;Acc:MGI:2444086]
Coordinates
chr7:119538085-119538389:+
Coord C1 exon
chr7:119538085-119538165
Coord A exon
chr7:119538166-119538287
Coord C2 exon
chr7:119538288-119538389
Length
122 bp
Sequences
Splice sites
5' ss Seq
CAGGTAGGC
5' ss Score
9.88
3' ss Seq
GCTATGACCCCCCCTTTCAGATT
3' ss Score
8.78
Exon sequences
Seq C1 exon
GTTGTCATCTGTGGCAATTCAAGAAACTCGACAATCAAGTCTGGATCTATGGGGAAAGCAAGCCCACCTTACGATGTGCAG
Seq A exon
GTAGGCAGCCCCTGCTGACAGTGCACAGAGCAATTTATGTTCTGGGGGATACAGGGTGGGCTTTTGGGTACCGTCACTGTGTAAAGGATGCTCAGATGTCATGCTATGACCCCCCCTTTCAG
Seq C2 exon
ATTGTAGATGAGGAGGGCAATGTCCTGCCTCCAGGAAAAGAGGGAAATATTGCTGTCCGAATCAAGCCTACTAGACCCTTCTGTTTCTTCAACTGCTATCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000030972:ENSMUST00000066465:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact

ORF disruption upon sequence inclusion

No structure available
Features
Disorder rate (Iupred):
  C1=0.169 A=NA C2=0.203
Domain overlap (PFAM):

C1:
PF0050123=AMP-binding=FE(6.3=100)
A:
NA
C2:
PF0050123=AMP-binding=FE(8.0=100)


Main Inclusion Isoform:
NA


Main Skipping Isoform:


Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
TTGTCATCTGTGGCAATTCAAGA
R:
TAGCAGTTGAAGAAACAGAAGGGT
Band lengths:
178-300
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Pre-implantation embryo development
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types