DreINT0025575 @ danRer10
Intron Retention
Gene
ENSDARG00000004880 | acsm3
Description
acyl-CoA synthetase medium-chain family member 3 [Source:ZFIN;Acc:ZDB-GENE-080220-22]
Coordinates
chr2:44879930-44880319:-
Coord C1 exon
chr2:44880239-44880319
Coord A exon
chr2:44880032-44880238
Coord C2 exon
chr2:44879930-44880031
Length
207 bp
Sequences
Splice sites
5' ss Seq
CAGGTCCAA
5' ss Score
-1.33
3' ss Seq
CTGGACTGTTCTGGTTTAAGGTG
3' ss Score
6.62
Exon sequences
Seq C1 exon
GTTTTAATAGCGGGCACATTCAAAGGCATGAAGATCAAACCAGGATCTTTTGGAAAAGCATCACCAGGATATGACGTGCAG
Seq A exon
GTCCAAAGTTTAAGCTAGACATAATAAAAAAAATCATTGACTGCTTGTAACGTAAAGATGTGTTCAAGTTGTCTTATAATTTGAGTTGATTCAACTTAAATCAAGTCAAGTAAAAAAAAAGCTGTGCTAAAAGTTGCCTTACAATTTAAGTTACAAGTTTTTTGAAGTTGGGCTGCTAATAGTAATTCTGGACTGTTCTGGTTTAAG
Seq C2 exon
GTGGTGGATGAAAATGGCTCTGTTGTGCCAAAAGGACAAGAGGGAGACCTCGGCATCCGAGTGAAACCAGAGAGACCCTTCTCTCTTTTTACAGAGTATACG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000004880:ENSDART00000041806:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.088
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(6.3=100)
A:
NA
C2:
PF0050123=AMP-binding=FE(8.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTTTTAATAGCGGGCACATTCA
R:
ATACTCTGTAAAAAGAGAGAAGGGT
Band lengths:
180-387
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]