BtaINT0029325 @ bosTau6
Intron Retention
Gene
ENSBTAG00000012210 | C5
Description
complement C5a anaphylatoxin precursor [Source:RefSeq peptide;Acc:NP_001160088]
Coordinates
chr8:112311982-112314128:-
Coord C1 exon
chr8:112314014-112314128
Coord A exon
chr8:112312109-112314013
Coord C2 exon
chr8:112311982-112312108
Length
1905 bp
Sequences
Splice sites
5' ss Seq
GCGGTAAGA
5' ss Score
9.63
3' ss Seq
TATTTTCCCCCTTTAAAAAGTTG
3' ss Score
4.93
Exon sequences
Seq C1 exon
ATATTTTTATAATAAAGTCGTCAACGAGGCTGACGTTTACATCACTTTTGGAATAAGAGACAACTTAGAAGATAATCAAAAAGAAATGATGCAAAAAGCAATGCAAAGCACAGCG
Seq A exon
GTAAGAGATTCACTTGTACCTAAAGATCCGTCATGAACGCTAGAGCTGGAAAGTCACTTCTGTATCATCTAGCCCACGGTACTGTTTCATGTAGGTGTGGAAATTGAAGCTTAAAAACCTTCCATTGACTTCTCACTATCCTGAGGATAAAGTTCTGTTTCCTTGTCACCAAGCCCCCTGCCGGCCCATCTCTCCCTTTCTTCCCCCTTGCGCTGGTCACCCCAGTTTTGCAAACTCCCCCCAGTCTCCCTCGCCTCCAGGTGTCTGCAGAGCTGCTCCTTCGGCCAGGGAATGCCCTTTCCCGTTGTCACCTGCTGCCTCTTCTCTGCCTGGCTAAGCTGTCCCGTTCTTTGTCTTCACATCATCACCCATCCTGCCGCCTCAGGCCTTGAGTCGCTCTCGGTGGAGCGTGTTTCACACTTTATATTGCAGTGGTTTTCAACCAAGGGTGATTTTTACCCCCAAGGAGGCATTTGGTAATGTCCAGAGACATTTGTGGTTGTCACAACTGGGCAAGAGTGTCACACGAGCATTTCGTGGGTCGAGGCCAGGGATGTTGTGAAACTCCTTCAGAGCAGTGCTCCCCAACAATGAATTACCCTGTGCAAAATGTCAATAGTGCTGAGGTTGAAAAACCATTACAGTTAGTAATGCTTTATTTACTCTATCGTTCTCTACACAAAACTCTTTGGGGGTCTGAATCATTCCCACTATATGCCTAGAGCCAGCTTGGTTGCTGGCAAATTGTGCAGGAGCTCTATAAAAATTTGTAAGTGAGTGAATTAACCGTGAGCTTGCTGAGAAGCTGTCGGCCACAGTCAAGGCCCAGTGGCTGCTGGAGCTCAGAGTGCCCTGTGGCCCTCGCCCCAGCTCAGTGCTCTCTGCGTCGCCCTGGATGGTAAGGGCTCAGGTCATTTTGTCCACTGCTGCACTTTCACAGCAGAGAAGGCAGTGGCACCCACTCCAGTGCCCTTGCCTGGAGAATCCCATGGATGGAGGAGCCTGGTGGGCTGCCGTCTATGGGGTCGCACAGAGTCGGACACGACTGAAGCGACTTCGCAGCAGCAGCTACACTTTCACAGAGCTTCTATCACCAGTTACTGAGCCCTTGCTCTTTCAAAGTACAGTTTTATTTAAGATATAAACGCCTCTTTTTTAATGCAAGATTCTATGTTAAACACTGAGGTTTAAAGCTACATAAGACAAAAATCCCTGGCCTCGAAGTACTCGATTTTAGAAGAAAAAATAAGGCAAAATCTGTCTTACTGTGTGGTTAATAATTAAATAATATCATATGCTTCGCTGGTGGCTCAGACAGTAAAAATCTGACCAGGGTTCGATCTCTGGGTCGGGAAGATCCCCTGGAGAAGGAATGGGAACCCACTCCAGTATTCTTGCCTGGAGAATCCCATGGACAGAGGAGCCTGGCAGGCTGCAGTCTGTGAGGTGGCAAGGAGTCGGACACAACTTAGCAATTAACATATCGTGGATAACCCTATATCCCATTATCCACTGTGCGTTTTTAAATTAGTGTCTTAATATGCAAGCAGTAAAGTACAGAGTAAATATCATAAAATCCCATAAACTCTCAGTTAAGAGCAAAAGTAGGCTGAGAGGCCTAGAAAGATGATGATGACTCAGTGATGGGAAGAAGTCATCTTTGCCAGCTAGTACAAAAAAGACCTGTGGAACCAAATGATGTTTAAGATTCTTCCACATCTATAATTCTAAACAAATAGATTAGAAGATAACACTCTGGGCTTAACTGTGGACTCAGTTACTCGCGTTAGTGCTAAAGCTTTCGTCTTTGTCTTTGATTGAGAGGCTAGTAAAGGCATATTTTATTCATTTTTTGCTTATAAATTCTCCTCTGAAATAATTTGTTTATTTTCCCCCTTTAAAAAG
Seq C2 exon
TTGATAAACGGAATGGCTCAAGTCACATTTAACTCTGAAACAGCAGTTAAAGAATTGTCCTATGAAAGTCTAGAAGATTTAAACAACAAGTACCTCTATATCGGTGTGACAATCATAGAGTCTACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000012210:ENSBTAT00000016204:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACGAGGCTGACGTTTACATCAC
R:
AGACTCTATGATTGTCACACCGA
Band lengths:
215-2120
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]