BtaINT0046194 @ bosTau6
Intron Retention
Gene
ENSBTAG00000018365 | CYP2E1
Description
Bos taurus cytochrome P450, family 2, subfamily E, polypeptide 1 (CYP2E1), mRNA. [Source:RefSeq mRNA;Acc:NM_174530]
Coordinates
chr26:50552443-50554655:-
Coord C1 exon
chr26:50554514-50554655
Coord A exon
chr26:50552631-50554513
Coord C2 exon
chr26:50552443-50552630
Length
1883 bp
Sequences
Splice sites
5' ss Seq
AAGGTAGGA
5' ss Score
9.45
3' ss Seq
TTCCATGCATATCTTGGCAGAGA
3' ss Score
5.07
Exon sequences
Seq C1 exon
GAAAGACACAGTGTGGACCCTATGTACACCTTGGAAAACATCGCTGTGACTGTGGCCGACCTGCTCTTTGCGGGGACAGAGACCACCAGCACCACCCTGCGATACGGGCTCCTGATTCTCATGAAATACCCGGAGGTTGAAG
Seq A exon
GTAGGACAAGTAATAGGCCAGGACTCTGGACATTGTGGCTGTCACACGTGGACGGAGACCCTGTGTCCCTCAGCCTGGAACATTCAGGCTTCAGCTCCCTGTCTGCAAAACAGGGAGTGACACTGGGGTCACTGGAGGAACGCTCTGGGGGAAGTGTTCTTGTACATGACAGGCACCTGGCACTCAGCCCTCCCACACCCACGGACACACCCACATTGTGAAAGAAAGTCCAGTGAAGAAACGCCATACTTCCAGAATCCACTCCAGGACATGATCTGTTTATGAGAGAGTGAACATGTGCTCATCACCCTAAGACCCAGCCTTTCAGGAAGAGCTGGGAGGGAGGAGCAAGACCTCCTGGCTTATCTCACCAGATTGGAAGAGGGATCACAGAAGGAACCTCTGGTAAAGCATTGCAAGTCTGCCTGCTGCCCTGGGTCCAGCCCTCCTTTCCAGGGCTCCTCCCCAGCACCTGAACCAGGTGAACTTGGGGTAGATCAGGCGTGACTTTGGGGGCTGCATGCGCAGAGCGTCAGGCATTAGAAACACCCCCACCGCAGCCTATAGGTGAGCAGCACTGTATGTTAGCAGGCTGGTCAGCACCGATACTAAGGGAAGAGGAGGCAGAGGGGCCGTCACCAGTGCTCGGGCCTGGAGCCCCCCACCTTTCACTGGGCTGCCCAGAGCTGCGGTGGGGGTCCCCACTGCAAGGAGCTCCTCACCACCTCAGCCATGCCTCTTGCTGATGATAATAATTTGGTTCCTGCTGTGTGCCAGGTGTGGGCATTAGCCCTTTAATACACTGTGGTCTTGAAGGCTGATGTGAAAGGGCATGAAATTATTACCTCGGGAACATGGGTATGTGACCCATCCAGGGTCAGACCTGCTCCATCTGACTCCAAGGCCTTCACCTTTCTATTGGACAGAAAAGTAAGGGAAAGGAGCCCAGAGTCCACAGAGCAAAGCCCCTATGCATCTGGGGCAGTTTTGAAGGAGCTCTGACCACTCTGTACTAGGAGATCCACAGCCTCCAAAATTGTTTTATTTTATTAATTATGACAAATTTCCAGTATAGAGGAAAAGACAGAAAATAAATATGACAGTCATCTATACATCCATTGCCAAATTTAACATGTTAACGTTTTGGAGTACTGACTCGGAGCTCTTGAAAAGAAAGATTGCAGAGCCAGCTGAGGATGCTCCTCACCTCCCTGGCTTCCCCGCCCGCCCCAGACTGCAGTCTTCCTGGAGTGCTGTGCAGCATCCCATGTAGGGCTTCTTACATTTACCAATTACTAAGTATTTACCATTTGCTAAGTAATACCATCTGTATTGTTATATGTGTTGAAATTTTTTCACTTGGTTACAATACTGCAGGGCGCTGTGTAGTCCTCTCAACATATTACAAAGGCCCACAGTTAACCTCAAACCTAGCAAGGCCAAGCAACTGCCAAAGGCTGCTGTTGCTTTTGCCAGGAGAGGGGCTTCCAGAATCCTTCCTCCAAGGCCCTTCAGGAGTACGACATGCTTGTCTGTTTCCACTCTCCATTTGCCTCCATACTCCGAAGAAGCATGCAACAAGCCAGCCTGGGGTAACAATTAATACAGTTTTCCTCCTGCTAGAAATCCCCAAGACCCTTTTTAATCCTCTCCTCCTGGCATGGAGGCAAGCACTGGACTAGCCATGACCAGCTAAGAGAATGGAGTCCATAAATAGACATGTAAATGCTGTAGACGGCACGTAACTTCACTGACCTTAGCTCTTGCTGACATGTGCGGATGAATTACCCAATAATCTGAAGCTCCATAAACATCTACTGGGTCTATGGATGGGTGTTGAGCCAGCTTGGAGTGATGCATTTATTCCATGCATATCTTGGCAG
Seq C2 exon
AGAAACTTCATGAAGAAATTGACAGGGTGATTGGGCCAAGCCGAATCCCTGCCGTCAAGGACAGGCTGGACATGCCCTACCTGGATGCTGTGGTACATGAGATTCAGCGATTCATCGACCTCCTTCCCTCCAATCTGCTCCACGAAGCAACCCAGGACACAGTGTTCAGAGGATATGTCATCCCCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000018365:ENSBTAT00000024437:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006717=p450=FE(10.3=100)
A:
NA
C2:
PF0006717=p450=FE(13.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAAAGACACAGTGTGGACCCT
R:
ACATATCCTCTGAACACTGTGTCC
Band lengths:
320-2203
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]