BtaINT0065969 @ bosTau6
Intron Retention
Gene
ENSBTAG00000000730 | GCG
Description
Bos taurus glucagon (GCG), mRNA. [Source:RefSeq mRNA;Acc:NM_173916]
Coordinates
chr2:34404410-34405408:+
Coord C1 exon
chr2:34404410-34404539
Coord A exon
chr2:34404540-34405270
Coord C2 exon
chr2:34405271-34405408
Length
731 bp
Sequences
Splice sites
5' ss Seq
AAGATGAAA
5' ss Score
-3.67
3' ss Seq
AACTTTCACATTTCTTTCAGGAA
3' ss Score
9.45
Exon sequences
Seq C1 exon
TTGGTGAAACAGGAGAAATTACAAATTTGAAAAAATGCAAACTGTCTTCATTTCTAGAGAGACAATGCCACAACTTGGCGAATGAATGTAAAAGAATCCTAGCTCCCAGGTGGTTTCTGTTGTGTTTAAG
Seq A exon
ATGAAACCTTCCAGGACTTATATATGATGGAGCAACTGACCATCTTTCTGGTCAACCCCTTCCACGGGGCATTCTGTACACATGTGAGGGTGATGTAAGGTCATTTTCTTAGCTTTCCTCACTGCTAGCCCATCATACATTTCCTTTAATTCATTATCCTGTGGTCCTTTCAATGACATTTTGTGACGCTCCTTTGCTTTCCTTTGATTCAATAAACTAACTCTGACCCCATGTGTTGGAATAGGAAATAGAAACCCACTCCAGTATTCCCGCCTGGAAAATTCCATGGACAGTGGAGCCTGGCAGGCTACAGTCCATGGGGTCACAAAGTGAGTGACCGAGCACACACACACGACCCTATATGTACAAATGACATGCTGCTGCTTTGTTTGCGAGTGGCATGGCACAGTAGATAGCCCTCACACTTTTGGACTGAAGTAAGGGCAGCGTTAAGAATTAATGATACAGACAAGCAGAAGAGGTGAAAATGGGTCACTGGGTGGAGTGAGGGAGCTTAGCCTCCCTTAATTGTCATTAGATTGCCCCCATCAATCTTCACTCCCAATAATTAATGCAGCTTTCTGATGAGTCTCCTACACACCCTCATCTCCCCAATCTCCCTCTAACCCCCCTCCACACAGAATCCAGAACCCGAAGCTGCCATTGCTTATAGGTGAGAACCATATTGCTAAAGAGACAGATCTTCAATTTAACTTTCACATTTCTTTCAG
Seq C2 exon
GAATAACATTGCCAAACGTCATGATGAATTTGAGAGACATGCTGAAGGGACCTTTACCAGTGATGTAAGTTCTTATTTGGAAGGCCAAGCTGCCAAGGAATTCATTGCTTGGCTGGTGAAAGGCCGAGGAAGGCGAGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000000730:ENSBTAT00000064745:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.106
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0012315=Hormone_2=WD(100=59.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTGGTGAAACAGGAGAAATTACAAA
R:
CTTCCTCGGCCTTTCACCAG
Band lengths:
262-993
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]