MmuINT0068110 @ mm9
Intron Retention
Gene
ENSMUSG00000000394 | Gcg
Description
glucagon [Source:MGI Symbol;Acc:MGI:95674]
Coordinates
chr2:62314881-62316773:-
Coord C1 exon
chr2:62316612-62316773
Coord A exon
chr2:62315019-62316611
Coord C2 exon
chr2:62314881-62315018
Length
1593 bp
Sequences
Splice sites
5' ss Seq
CCGGTAAGA
5' ss Score
10.1
3' ss Seq
AGCTCCCGCATTTCTTTCAGGAA
3' ss Score
8.89
Exon sequences
Seq C1 exon
ATCATTCCCAGCTTCCCAGACAGAAGCGCATGAGGACCCTGATGAGATGAATGAAGACAAACGCCACTCACAGGGCACATTCACCAGCGACTACAGCAAATACCTGGACTCCCGCCGTGCCCAAGATTTTGTGCAGTGGTTGATGAACACCAAGAGGAACCG
Seq A exon
GTAAGAGTCTGAAGTTGTTGCACACAACTTCATATACATATTAACTTCAGTACATATTAAGAAATTTTCCCGACTCCCCTAGGTTATCATGCTCACCAAACAGGCTTATTCATGTTGGATATTTCTGATGTTGACCGTGCTACCCCTTGACTTTGCTCTATAGAAATCCTTATCCACACACAATTGTGACAAAAACATTTTATTATCTTAAACTGCAGTGTTTTTAGAAATTTTCTAAACTGGAAACTGAGTTTTTATGACCTGCTAGGTTGAGAATGTTATCTTACTAAAAGTTCGAGAAAACTCACAACATGCAATTGAGGGGTCCAAGTCAATGACTGTGATCCGATTAAGATGAGACCTTAGCTGTATTGATCGTTGGTAAAGCACGAAAAGCAGCTGCATCCATTCTTTTGTAACATAAAATCCAAGAAGTGCATTTAAAATCACAGCCTAGCTAGTTGCTGAGTGTAAAGAGAGAAGGTGGCAAGCAGGAACACAAACCCATCATAAGGCATACATTTTAATGTTGGATTTTGTTTGAGGAGTGTTGGGGGCTTTGCTACTTCTAGGCTACTGGAGGAAATTATCCACAAGAGTTTCGCAGAGATGGTGGAATTTCACAATTTCTTTGGAGGATAGCCCACATGGAAAAGGAAGTTATGCATACCAAGTAGCTGAGGAAATCTAAAAATCAGGAAAAGGACATAGATATATTTGGGTGGGATGAATAGGATAGGAAGAGGGTATGAGACAAGGAGACAGGGCACATATATCCTTGGAGTGGAGAGTAGGGCAGAAAAGATACCCATAGTGGTCAGAGCAGACCTGAGGGCTGACAATGTCCCTGCCAGTGACTTATAGGTAAGGCATGACTGTGAGAAAACACAAATTTATAGACTTGAAAACCATTGTTCTGATTTTTCTAGAGTGGAAATGATACAATGTGGGCAAACTAACACAAAGCAAGAGACCCATAACCTCAGCTATTTGTTGTCTGATTAAGATGAACGTTCCAAAAGGGCGATGTACAGCACGGGTCTTCAGGCTGCTGGTTCTGTCGCCCCTGGGTTCTTCGAACACGCTTGCAGCTGATAAGAGCCCACTTTGCTTCTCTCCCGCTAGAGCATTTACTTTCTTTAATCCGTCATCATATGGTTCTTCTCATAAAAATTCATGACAGTCCTTAGCTTTCCCTCGGATCGGTTAACTGACTCTGACTGGCTGTTCATTTAATAACAAGTACCTACTCTGACTGTGAGCAACATGGCGGGGTGGGCAGCCTTCCTGTCCTAAAATCAAGTAAAACGTAAATAAAGCTTTAACAACAATGGCATGAGGAAGCAGAGGAGGTGAAAATGGCCCCTGGAAAAGGAAGGTTTAGTGTCTTTTAAAATGTCATTAGCCTTTCCCCACAAAGTTTCAATCCAGATCATTAACAGAACTTCAAAATAATTGTGTAACCGCTCTCACCTACCAAACTCCTTAGCCCTCTCTCCTTTCAGTCCTGAGCCTAAGGTTCGCACAGCTTATCAAGGAGAAACACACTAGCAAAGATGCAAAGCTTCAATTCAGCTCCCGCATTTCTTTCAG
Seq C2 exon
GAACAACATTGCCAAACGTCATGATGAATTTGAGAGGCATGCTGAAGGGACCTTTACCAGTGATGTGAGTTCTTACTTGGAGGGCCAGGCAGCAAAGGAATTCATTGCTTGGCTGGTGAAAGGCCGAGGAAGGCGAGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000000394-Gcg:NM_008100:3
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.473 A=NA C2=0.213
Domain overlap (PFAM):
C1:
PF0012315=Hormone_2=WD(100=50.9)
A:
NA
C2:
PF0012315=Hormone_2=WD(100=59.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCATTCCCAGCTTCCCAGACA
R:
CCTTCCTCGGCCTTTCACCAG
Band lengths:
294-1887
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: