BtaINT0065991 @ bosTau6
Intron Retention
Gene
ENSBTAG00000032288 | GCK
Description
Bos taurus glucokinase (hexokinase 4) (GCK), mRNA. [Source:RefSeq mRNA;Acc:NM_001102302]
Coordinates
chr4:77847630-77848492:+
Coord C1 exon
chr4:77847630-77847792
Coord A exon
chr4:77847793-77848337
Coord C2 exon
chr4:77848338-77848492
Length
545 bp
Sequences
Splice sites
5' ss Seq
CAGGTACCG
5' ss Score
10.05
3' ss Seq
ACTTCTGTCTGTGGCTGCAGAAG
3' ss Score
7.61
Exon sequences
Seq C1 exon
GCGGAACAGATCCTGGCAGAGTTCCAGCTGCAGGAGGAGGACTTGAAGAAGGTGATGAGGCGGATGCAGAAGGAGATGGACCGAGGCCTGAGGCTGGAGACCCACAAGGAGGCCAGTGTGAAGATGCTGCCCACCTATGTGCGCTCCACCCCAGAGGGCTCAG
Seq A exon
GTACCGTGGTCCAGCACAGGGCTGGGATGGAACCCACATGGGAGCCAGCAGGTCCTCAACCAGAGGCAGCTGTCCCCTAGCTCCAGCAGGGAGAAGTTCCCTAGGCCCTTCTTAACAGCCCTGACTGTCCCTAGCAGCCCCAGCGTGCTGGGCCCCAGACACTGGCCTGTTCTTACTCTTGGCTCCGTTGGCTCAGCCCTGGGGCCTTCAGGTTCATTCTGACCTCAGAGAAACTGGTTTTTTTCATTTCACAAGTAGAAAAATGGTTTCCTATAGTCATTTGGGGGACTCTCAGCCCAATCCTGAGTCTGGACATTTTCCTAGATCTTCGGGTGGGCTCTGGGCTTGGGGGGAGTTCAGTCCAAGGCAAGGAATTCCTGCTGACTTACCCAGTTCACCTTTGACCCTTTCCACAGTTTACTCTGGGGTCCGGTACCAGGGGCCCCTCTGGTGCCCCCACCATGCCTTTACCTCCTCTTCTCCAGTCTCGGGGGTCCTGCCAGGCGGTGCCCATGAGACTGATGTACTTCTGTCTGTGGCTGCAG
Seq C2 exon
AAGTTGGGGACTTCCTCTCCCTGGACCTGGGTGGCACCAACTTCCGGGTGATGCTGGTGAAGGTGGGAGAAGGCGAGGCGGGACAGTGGAGTGTGAAGACCACGCACCAGATGTACTCCATCCCCGAGGATGCCATGACGGGTACTGCTGAGATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000032288:ENSBTAT00000045734:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.164 A=NA C2=0.019
Domain overlap (PFAM):
C1:
PF0034916=Hexokinase_1=FE(26.1=100)
A:
NA
C2:
PF0034916=Hexokinase_1=FE(24.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCGGAACAGATCCTGGCAG
R:
CATCTCAGCAGTACCCGTCATG
Band lengths:
318-863
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]