BtaINT0074562 @ bosTau6
Intron Retention
Gene
ENSBTAG00000014093 | IDH2
Description
Bos taurus isocitrate dehydrogenase 2 (NADP+), mitochondrial (IDH2), mRNA. [Source:RefSeq mRNA;Acc:NM_175790]
Coordinates
chr21:21905039-21905421:-
Coord C1 exon
chr21:21905309-21905421
Coord A exon
chr21:21905137-21905308
Coord C2 exon
chr21:21905039-21905136
Length
172 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAGT
5' ss Score
10.47
3' ss Seq
CCTGCGACCTCTTCCTCCAGGGC
3' ss Score
10.57
Exon sequences
Seq C1 exon
GCTTTGGCTCCCTTGGTCTGATGACGTCCGTGCTGGTCTGCCCGGATGGGAAGACCATTGAGGCTGAGGCTGCTCATGGCACAGTCACCCGCCACTATCGGGAGCACCAGAAG
Seq A exon
GTGAGTGCCAGGGCGGTGGCTGTGTGCCCTTCTATCCCTGGAGCCCAAGCCCACTGGCCCTGAGGGCTGGGTGCATGGGCCTGGTCCTGTCAGTCTAGAGGGGGCATCTGTGTCCAGGGGGCCTTTTCATTGGCTGCTTGGCTCTTCATCCCCCTGCGACCTCTTCCTCCAG
Seq C2 exon
GGCCGGCCGACCAGCACCAACCCCATCGCCAGCATCTTTGCCTGGACGCGTGGCCTAGAACACCGGGGCAAGTTGGATGGGAACCAGGACCTCATCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000014093:ENSBTAT00000018741:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.237 A=NA C2=0.061
Domain overlap (PFAM):
C1:
PF0018015=Iso_dh=FE(9.3=100)
A:
NA
C2:
PF0018015=Iso_dh=FE(8.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTTTGGCTCCCTTGGTCTGAT
R:
CTGATGAGGTCCTGGTTCCCA
Band lengths:
210-382
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]