RnoINT0072863 @ rn6
Intron Retention
Gene
ENSRNOG00000013949 | Idh2
Description
isocitrate dehydrogenase (NADP(+)) 2, mitochondrial [Source:RGD Symbol;Acc:1597139]
Coordinates
chr1:141875336-141875672:-
Coord C1 exon
chr1:141875560-141875672
Coord A exon
chr1:141875434-141875559
Coord C2 exon
chr1:141875336-141875433
Length
126 bp
Sequences
Splice sites
5' ss Seq
AAGGTAAAT
5' ss Score
8.88
3' ss Seq
ATCCCGTGACCTGTCCCCAGGGC
3' ss Score
8.24
Exon sequences
Seq C1 exon
GATTTGGCTCCCTTGGCCTGATGACATCTGTGCTGGTCTGCCCTGACGGGAAGACCATTGAAGCTGAGGCTGCTCACGGGACAGTCACCCGCCACTACCGAGAACACCAGAAG
Seq A exon
GTAAATGTGTGCACTTCAAGGTCCAAATCCCATTGGCCCTGAGGGCTGGGAGGATGAGCCTGCTCTGCAGGTCTGGAGGTACACAGCTGTATCTGGTGCTCGGCTGATCCCGTGACCTGTCCCCAG
Seq C2 exon
GGCCGGCCCACCAGTACCAACCCTATTGCCAGCATCTTTGCCTGGACACGGGGTCTGGAGCATCGAGGGAAGCTGGATGGGAACCAGGACCTCATCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000013949:ENSRNOT00000019059:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.237 A=NA C2=0.061
Domain overlap (PFAM):
C1:
PF0018015=Iso_dh=FE(9.3=100)
A:
NA
C2:
PF0018015=Iso_dh=FE(8.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATTTGGCTCCCTTGGCCTGAT
R:
CTGATGAGGTCCTGGTTCCCA
Band lengths:
210-336
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]