BtaINT0082797 @ bosTau6
Intron Retention
Gene
ENSBTAG00000009501 | KLKB1
Description
Bos taurus kallikrein B, plasma (Fletcher factor) 1 (KLKB1), mRNA. [Source:RefSeq mRNA;Acc:NM_001046352]
Coordinates
chr27:15342779-15343946:+
Coord C1 exon
chr27:15342779-15342954
Coord A exon
chr27:15342955-15343850
Coord C2 exon
chr27:15343851-15343946
Length
896 bp
Sequences
Splice sites
5' ss Seq
CTGGTACGT
5' ss Score
9.57
3' ss Seq
ATGTAAAAATTACCTTTCAGATT
3' ss Score
7.08
Exon sequences
Seq C1 exon
GCTTCTCTTGTCAAATATTTGGCGCATTTATGGTGGCATTTTAAATCTGTCAGAGATAACGACAGAGACGTCTTTCTCACAAATCAAAGAGATTATTGTCCACCCAAATTATAAAATCTCAGAAGGCAGTCATGACATTGCCTTAATAAAACTTGAGGCTCCTTTGAATTTTACTG
Seq A exon
GTACGTAGCATGTTTAAGGAGACATGTATAGCTGAACTGTGTTGGTTTAAATTTTCACATCAGTTTCAAGGAGGGGTCAGAATCTAAGGAGACTGTCTTTATTTTCTGGTTAATGAAGTTGCGGGAAAGATGAGTATTGCCAGGAAAAATAATTTGAGACTTGACATAAAATGTCTTCTACTTAAGGGCCAAGATGTGTTACTTAGTTCTTTCAGGAAGGAATACAGCTGCATATTATACTATTGAAATAATAACAGACATGGAAGGGAGCATGACTTCCTTTGACAAGAGTAGCCGGATTTGCATTGCCTTGAGCATAGGCATGTTAGGAGTATCTGAGGGCAAGCATATTCAAATATTAATTTCCAGGTGAAATCTGAGTAGGGAAGAGACTGCTGGACAAGGCAGTTCTGGGAGACAGTAGCTTTGTGGTTGGACGGGGTGCATTTGGAGGTACAAAAAGCCCCACAGTTGCTAAATGCCTGCTGGTTCTGCTAAAGATTCTGTGTCCTCCGGCAGGATGTCCTTGCCTCCAGTTACCCCACCATGGGATTCTTATTAAAATCAGTTAATAAGTTAAAGTGCCTAAAAATCATGCTTAAAACAGTGTAGACCCCTCTGCTATTACTATTGAGGGAATCATACACAAGAACTTGTAAAGGCTCTTGACAGTAAATTTGGGTTCGGAGCTTTAAAATTTAACACCCAAAATGACTGTGATTGGTGAGACATTTTCTTGAGTCACTGACCTTAGAAACTGAGTGGAAAGTAAAAGGTGTATTCTACTCGGTGCTACTCTAGAGAAAGAGAATATAGGGGCAGGGAGAGTATGTCTTTTTAAAGTCAATGTCCGTAACTAAATCTAGCCAAACTCTAATGTAAAAATTACCTTTCAG
Seq C2 exon
ATTTGCAAAAAGCAATATGCCTACCTTCCAAAGATGATACAAAACCAGTTTACACTGACTGTTGGATAACTGGATGGGGCTTCACAGAGGAAAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000009501:ENSBTAT00000012503:12
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0008921=Trypsin=FE(25.5=100)
A:
NA
C2:
PF0008921=Trypsin=FE(13.9=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTCTCTTGTCAAATATTTGGCGCA
R:
CTTTTTCCTCTGTGAAGCCCCA
Band lengths:
270-1166
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]