BtaINT0088374 @ bosTau6
Intron Retention
Gene
ENSBTAG00000021024 | MACF1
Description
microtubule-actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664]
Coordinates
chr3:107333752-107334875:-
Coord C1 exon
chr3:107334721-107334875
Coord A exon
chr3:107334029-107334720
Coord C2 exon
chr3:107333752-107334028
Length
692 bp
Sequences
Splice sites
5' ss Seq
TAGGTGAGC
5' ss Score
7.96
3' ss Seq
TTTCTCTTCTTCCATCTCAGGGT
3' ss Score
10.98
Exon sequences
Seq C1 exon
GATGCTGCTCACCTGGAGTCAGGGCAGCCAGTGCAGTGTGAGTCAGATGTCATCATGTACATTCAGGAGTGTGAAGGTCTCATCAGACAGCTGCAGGTGGATCTCCAAATCCTACGAGATGAGAACTACTACCAGCTAGAAGAGCTGGCTTTTAG
Seq A exon
GTGAGCAACATGGGACTCAAACAGAGGGCCTGATCACTACTGGTTCTCAGATATTATATTAAGAGTCGGGCACTGAATCTTACAGCCTGGACGTTAGAGATCAGTGTCTCAGTAGAGATGCTACAGGTATTTGAGGCAGGAAAATTCTCATGGTAGAAGCAGGTTTCCTTTATTTGAGGCCCTGCCCACTAAGTACCTGAAGGATTCCCTAATGTTTGTGTATGGCGTCTTATAATATCCCCACATATTTCAGAGGGACCTGCAGCACTGTGGTACCACATCTGCTTTAGAACTACTGGAGTGATTCAATGTAAATTTTCATAGAGGAGGAACCCAGCGCATATGGAGGGGATGAGGATCTATGGACACACCTGAGACTAACAGCTACATCTTTTGACCCCTTAGTTCATGGTTTATTCTGTTGTATCATCCAGGAGTTGTAAACACTGGTTTTGATTTGCTTCCCTCCAGGTAACACTGTAACTTGGCACTGGCCTCAAAGATGACTCTGAACCACGAAGATGTACATAACACCACCTTGCATTCACAGAACACTTTTTCTTTTTAAAAACAGTTTCCCTAAGATGATCTCATCTATATTTTTACCTCATCCTTGTCAAATGTATAGGGAAATTTTTATCTCAAAAAGCACATTATAAATTTAATGTCTTCTTTCTCTTCTTCCATCTCAG
Seq C2 exon
GGTCATGCGTCTGCAGGATGAGCTGGTCACGTTGCGTCTAGAGTGCACGAACTTGTACAGGAAGGGCCACTTCACTTCCCTTGAATTGGTTCCACCCTCTACTTTAACCACTACTCATCTGAAAGCAGAACCCTTGACCAAAGTAACCCATTCTTCTTCTACCTCCTGGTTCCGAAAGCCTATGACTCGGGTCGAACTTGTGTCTATCTCCTCCTCTGAAGATGAAGGAAATCTTCGATTTGTGTATGAACTACTGTCTTGGGTAGAAGAAATGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021024:ENSBTAT00000033270:12
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.065
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0043516=Spectrin=PU(13.7=14.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATGCTGCTCACCTGGAGTCA
R:
TCGACCCGAGTCATAGGCTTT
Band lengths:
350-1042
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]