BtaINT0088429 @ bosTau6
Intron Retention
Gene
ENSBTAG00000021024 | MACF1
Description
microtubule-actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664]
Coordinates
chr3:107226506-107227624:-
Coord C1 exon
chr3:107227383-107227624
Coord A exon
chr3:107226713-107227382
Coord C2 exon
chr3:107226506-107226712
Length
670 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAGC
5' ss Score
9.6
3' ss Seq
AAACTTTTTTCCCTCTTTAGGAA
3' ss Score
10.77
Exon sequences
Seq C1 exon
GTGAAGAGGTATTGCTCATTCAAGAGAAGCTGGATGGCATAAAGACTCGCTATGCAGACATCACAGTCACTAGCTCCAAGGCTCTCAGAACCTTAGAGCAAGCCCGGCAGCTGGCCACCAAGTTCCAGTCCACATATGAGGAACTGACCGGGTGGCTGAGGGAGGTAGAGGAGGAGCTGGCAGCCAGCGGAGGACAGTCCCCCACAGGGGAACAAATACCCCAGTTTCAGCAGAGACAGAAG
Seq A exon
GTGAGCTGACATCCCACACTGTAAAGGGAAAGAATTTTTCTCTTAGAATCCTGAAGTTCAAATTCTTGAGTTTTACACCATCTATCTTTACCTGAAATCTAAACCAAAAATTTCTGTCTGCTTTGTTTTCTGTTTATTCGTGGAAAATAAGTTAGGACAGGCCTATATAAGAGCATTTATCATGGTCTGAAGAAGAAAAAGATTGGATCTGAACACCCTAGGAAGCTTGCTTCATATGGAACATTTTAGAACTTACTGTCATAGTTCACTCTCTAAAAGAGTACCTCATAGTCACCTCTAATTCTCCTGCAGGACCTTGTGCAGTTCTAGATGCACAGAAATACAGTGCATTTGTTGAGGTAAATGAATGCCTATGATTAAAGCATTATTTATCATTTCTATTGAAGTAAGGAGAAAAAATACAAGAAATACCATTCATGTTACCCAGAGACAGTCTGGCCTAAAAAGTCTTGCTGACAGTTCTAAGCAGCTGAGCCTAAAGTCAGGCTGTGGAGTGACTGAAAAATCTGAGACAAGAAAAAAAATAAGTGAAGGAAGGAGGTCAGAGCCCTGCTGGGATGTGTGGTAGAGAGAGCATTTTCCACCCAGCACAGCTGCTAATGTGCTTAATTTAGCAAGAGATGGCCTGAAAACTTTTTTCCCTCTTTAG
Seq C2 exon
GAACTAAAGAAGGAGGTCATGGAGCACAGGCTGGTGTTGGACACAGTGAATGAGGTGAGCCGTGCTCTCTTAGAGCTGGTGCCCTGGAGAGCCAGAGAAGGGCTGGATAAACTCGTGTCCGACGCCAACGAACAGTACAAGCTGGTCAGTGACACTGTTGGACAAAGGGTGGATGAAATTGATGCTGCTATTCAGAGATCACAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021024:ENSBTAT00000033270:63
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.272 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(30.8=40.7),PF0043516=Spectrin=PU(41.1=54.3)
A:
NA
C2:
PF0043516=Spectrin=PD(57.0=88.4),PF0043516=Spectrin=PU(3.7=5.8)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAGTCACTAGCTCCAAGGCT
R:
CCACCCTTTGTCCAACAGTGT
Band lengths:
352-1022
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]