BtaINT0091711 @ bosTau6
Intron Retention
Gene
ENSBTAG00000020839 | MEGF6
Description
multiple EGF-like-domains 6 [Source:HGNC Symbol;Acc:HGNC:3232]
Coordinates
chr16:50801586-50802070:+
Coord C1 exon
chr16:50801586-50801714
Coord A exon
chr16:50801715-50801941
Coord C2 exon
chr16:50801942-50802070
Length
227 bp
Sequences
Splice sites
5' ss Seq
ACTGTGAGT
5' ss Score
7.94
3' ss Seq
CCAACCCGGGTCTCCCTCAGCCT
3' ss Score
3.79
Exon sequences
Seq C1 exon
CCTGCCCCGCCGGGCGCTACGGTGCCGCCTGCAGCCTCGAGTGCTCCTGCCACAACCACGGGGCCTGTGAGCCCACGTCGGGGGCCTGCCGCTGCGGCCCCGGCTTCTACGGCCAGGCCTGCGAGCACT
Seq A exon
GTGAGTTGTAGGGTTGGGCCACCGCACCCCCGGGCGCGCACTCCTCTGCTTTCCGCGTCACTCCTTGTGGCGGCCCGATGCGACGAGGCCTGACCCAGCTTCGGGGCAGAGGGCCACAAGGGGCAGGCGCTGGGCCTGGGTGGGAGGAAGCGGGCTCCAGCCCCCAGCCCGTCCTTTGCTCTCTCTGGGCAAACTGAGTCACGGGCTCCAACCCGGGTCTCCCTCAG
Seq C2 exon
CCTGTCCCCCCGGCTTCCATGGGGCTGGCTGCCAGCAGACGTGCCAGTGTCAGCATGGCGCCCCCTGCGACCCTGTCAGCGGCCAGTGCCTCTGCCCTGCTGGCCTCCATGGCCAGTTCTGCGAGCGGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000020839:ENSBTAT00000027771:33
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=PU(63.0=65.9)
A:
NA
C2:
PF0005319=Laminin_EGF=PD(34.8=36.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTGCAGCCTCGAGTGCT
R:
GCTCGCAGAACTGGCCATG
Band lengths:
228-455
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development