BtaINT0097066 @ bosTau6
Intron Retention
Gene
ENSBTAG00000018204 | MYH1
Description
Bos taurus myosin, heavy chain 1, skeletal muscle, adult (MYH1), mRNA. [Source:RefSeq mRNA;Acc:NM_174117]
Coordinates
chr19:30121066-30123405:-
Coord C1 exon
chr19:30123150-30123405
Coord A exon
chr19:30121309-30123149
Coord C2 exon
chr19:30121066-30121308
Length
1841 bp
Sequences
Splice sites
5' ss Seq
TCTGTGAGT
5' ss Score
7
3' ss Seq
TGTCTACCATCCCTTCTCAGGAA
3' ss Score
10.12
Exon sequences
Seq C1 exon
AGAGTCCATCTTCTGCATCCAGTACAATGTCCGTGCTTTCATGAACGTCAAGCACTGGCCTTGGATGAAGCTGTATTTCAAGATCAAGCCCCTCCTCAAGAGTGCAGAGACAGAGAAGGAAATGGCCAACATGAAGGAAGAGTTTGAGAAGACCAAAGAAGAGCTGGCTAAGTCAGAAGCAAAAAGGAAAGAACTTGAAGAGAAAATGGTGACTCTGACACAAGAGAAGAATGACCTGCAGCTCCAGGTTCAGTCT
Seq A exon
GTGAGTATCAGAAGCCATTGTCTACTATCTGATGCTAAAATTCCAATTCAGCAGCTACTAGGGGAGAGTCTAGATGGCACCTTATTGAGGGACTGATCACCAGTCTTTCCTAATTCCCATCCTCAAAGTGTTCCATAAAATTTCATATGCACTATTATGAACAGCTCTGTCGGAGGATCCTGTGCTGTTGAGAAGAAAGTAATAAAAGTGATTTTCTTTTGCCCCTGGTGTTTTTCCTAGCAGAATACGGGCAGGAAATAAAGTGATCTTTGCTCCCTAAGATTCTCCCCACTGAAAAATGCAAATAAGAACAGTGAAGAAAGAACAATATAGACAGTAAAGAGGGTGGAGACTCTATGATGAAAATGTACCAACTGGAAACCAATATTGTGAATTTTTAAATCTATGGCCTCCTGGCAACTTCCCTCTCAACTAGCTCTTTAGGAATCCTCCAGTACCTGAAAATATAAGATCATTGCCAAGATAATGGCAGCACTTTCTAAAAATAGTGTCAGTGAGGCAAAGCATGAGTTGATGCTCTTGTTGTGATGTCCTAAGAAGTCTCCAGAGGCTTGGGAAGAGATATTTGATGAACATCACTGTCTATCCCATTTCTGTGACTCAAATGACAAGAAAATTTCAGCTGCCACCAATGAAATCAATAACAGAAATATTAAAAGTTACTCTCTAGTTCTCTGAATTCAGAAGGGAATCTACATCGCTTCTAGGGAGTGCACACATTTTAGACACATAACTAGAAAAAATAAAAGTGAATTATAAATACTTGGGTTGGCACTTCCCAAGTCACAAGTTGTGACTTCCCTTATGGTTTATGGAGTTCAAGATTTTTCAAACTGAGGATGGATTTATCATACCTTCAAAAATCAAGGGATAACTACATGGGAATTAACTCCTCTCAGTATAATATGCTCCAACCATTTCTTTTTTTTTCATTTACTAAACCTTTCTGGAAAACAATGCTAAATAAAGTCAGCTAACTGAAATTATGGGAATCAGGTTGGGGCTTCCCTGGTGGCTTAGATGGTAAAGAATCTGACTGCAAACACGGGGAGTTATCATGTAGGGGAGTAAAAAAATTTCATTGACCCTCTTAGGGTTCCTTGGCTAGGTCTGAAAATTAAACTCACAAAGATTGATAGGAGAAAAACATACAAACTTTATTTAATTTTTATGTATATATGTAAGCCTGTATAAGAGAATGAAGACCCAAAGAAGTGACCAGAGCATGAAGCTTTAATACCTTTGAGACAAAGAAATGATAAATACGTGAAGAACTGACAAGACAGGGGGTCCTGGACTGGGGTAGTAAGTGATGAAGAAACAGAAAGATAAGAGTTAGTTTAACAAGATTTGTTTATACAGCCTTCTCAGCCCTAAATTCCCCATCTCTTGTAATAAGAATGCCTTCTTTCCTCCCAGTACAGAGAGGGAACCTTCCATGTAGAAGGTTAGTGGCTTGTTTCAGGGAAAAGGGGCAAGAGGAATAGTTCAGAGTGGTCTTCCTGTTTCTGCTCTTTTCTCAAACTCCTTTAGCTTAAGATATTCATTGTGCCAAGGTACCATATTTTGGGACAGAATATCCTGAAACTCATCATTAATACGTTTATTTTATTATAATTTCACCAGGAATTGTAACTTAAATTCCTTTTTAAAAATCTCCAGTGCCTTAAATACCCAATAAATATTTCCTAAATTATGGAAAACTAATAAAAATTAACCTAAAGCTTTAAAGTACAATTTACTTATTTGGTTTCAATTCTTAATAAAATCTGAAAAGGTCCACAGAACAAGACCTAAAAGTGTCTACCATCCCTTCTCAG
Seq C2 exon
GAAGCTGATGCCTTGGCTGATGCAGAGGAAAGATGTGACCAGCTGATTAAAACCAAAATCCAGCTGGAGGCTAAGATCAAGGAGGTGACTGAGAGAGCCGAGGATGAGGAAGAGATCAATGCTGAGCTGACGGCCAAGAAGAGGAAACTGGAGGACGAATGTTCAGAACTAAAGAAAGATATAGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAACACGCCACAGAGAATAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000018204:ENSBTAT00000009327:21
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.256 A=NA C2=0.420
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=PU(27.4=62.8)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(40.6=100),PF0157614=Myosin_tail_1=PU(31.2=53.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAGCACTGGCCTTGGATGAAG
R:
CCAGTTTCCTCTTCTTGGCCG
Band lengths:
358-2199
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development