DreINT0100047 @ danRer10
Intron Retention
Gene
ENSDARG00000001993 | myhb
Description
myosin, heavy chain b [Source:ZFIN;Acc:ZDB-GENE-081124-1]
Coordinates
chr6:40752959-40753547:-
Coord C1 exon
chr6:40753292-40753547
Coord A exon
chr6:40753202-40753291
Coord C2 exon
chr6:40752959-40753201
Length
90 bp
Sequences
Splice sites
5' ss Seq
TCTGTGAGT
5' ss Score
7
3' ss Seq
GATGATATTATAATCAACAGGAA
3' ss Score
4.92
Exon sequences
Seq C1 exon
AGAGTCCATCTACTCCATCCAGTACAACATCCGCTCATTCATGAATGTGAAACACTGGCCATGGATGAAGCTGTATTTTAAGATCAAGCCCCTGCTGAAGAGTGCAGAGACTGAGAAGGAAATGGCAGCCATGAAAGAAAACTTTGAAAAGATGAAAGAGGATCTTGCCAAAGCCCTTGCAAAGAAGAAGGAGCTGGAGGAGAAGATGGTCACCCTGCTGCAGGAGAAAAATGACCTGCAGTTACAAGTGGCATCT
Seq A exon
GTGAGTTCACAAATGATCAGTCCATCTTTATAAGAGAACAAATACACAAGTTGGAAAAAAGACAACAAATGATGATATTATAATCAACAG
Seq C2 exon
GAAACAGAAAACCTTTCTGATGCTGAGGAGAGATGTGAAGGACTAATCAAAAGCAAGATTCAGCTTGAGGCAAAACTTAAAGAGGCAACTGAAAGATTGGAGGATGAGGAAGAAATCAACGCTGAACTTACTGCCAAAAAGAGAAAGCTGGAGGACGAGTGCTCTGAGCTGAAGAAAGACATTGATGATTTAGAGCTTACCTTAGCCAAAGTAGAGAAAGAAAAACATGCCACTGAAAACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000001993:ENSDART00000156295:22
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.047 A=NA C2=0.407
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0157614=Myosin_tail_1=PU(28.9=48.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGATCTTGCCAAAGCCCTTG
R:
TGCCTCAAGCTGAATCTTGCT
Band lengths:
170-260
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]