MmuINT0103504 @ mm10
Intron Retention
Gene
ENSMUSG00000057003 | Myh4
Description
myosin, heavy polypeptide 4, skeletal muscle [Source:MGI Symbol;Acc:MGI:1339713]
Coordinates
chr11:67250752-67251948:+
Coord C1 exon
chr11:67250752-67251007
Coord A exon
chr11:67251008-67251705
Coord C2 exon
chr11:67251706-67251948
Length
698 bp
Sequences
Splice sites
5' ss Seq
GCTGTGAGT
5' ss Score
7.39
3' ss Seq
TGTACTATTTCTCCCATTAGGAA
3' ss Score
9
Exon sequences
Seq C1 exon
AGAGTCCATCTTCTGCATTCAGTACAACGTCCGCGCCTTCATGAATGTGAAACACTGGCCCTGGATGAAGCTGTATTTCAAGATCAAACCTCTGCTGAAGAGCGCGGAGACCGAGAAGGAGATGGCCAACATGAAGGAAGATTTTGAGAAGGCCAAGGAGGATCTGGCTAAGTCAGAGGCAAAAAGGAAGGAACTAGAAGAAAAGATGGTAGCTTTGATGCAGGAGAAAAATGACCTGCAGCTCCAGGTTCAAGCT
Seq A exon
GTGAGTGTCGGATACTGCATATAGTCCTATCATAGTTCATAGTCAGGATGTGACTGCCTATAAGCAATCCCAGGGCTCAGAGTAAACAATTTCTATCCAGGCGCCTGTTGTGATTGCCTGCCCTTCAAACCATTAAGATTCAAACCCAAAGAAACAGCAGCCTAATGGTGCTTACTATGTGCATTGTACACAGCCTGGTTCATGGTGAACAAAGTCATGAAAACAGCTAGACATTTTCCTTTCTGTATACAACATCCTCTTATATTAGAATATTCTCTCTTTGGTAAATATAGGTAAATGGAACTAGAAAGTACATTATTTTTATCAGAGCAGGGAAAAACAAAAAACAAAAACAGGCTAATTTAAGCCTGTAATTGAATGTGAGGGGAAAAACCCTGAGTTCAAGAATTAGTAAATGACTCTTTCATAAGGACTGAGGGCTAGTAAGTCATTTGTATTTTCAACAAAGCCACTTTTTCCTCATTTTACCTTCTACACATAGATATTTAGAGTTAATATATTAATCTACTTGGTCAACATCTTGAGATTGGTGCATTTTGTCTAATTTTTTTTAAAGTTAGCAAGTTAATGGCCTACTCAAAGATCAACTGGTAGTCATCTGTTCCCAATTTTGAATACATTATAGTGTATTTTCGATATATCCTATACAAATGGAAGTGTACTATTTCTCCCATTAG
Seq C2 exon
GAAGCAGATGGCTTGGCTGATGCTGAGGAAAGGTGCGACCAGCTGATCAAAACCAAAATCCAGCTGGAGGCCAAAATCAAAGAGCTGACCGAGAGGGCCGAGGACGAGGAGGAGATCAACGCGGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCAGAGCTGAAGAAGGACATTGACGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACGGAGAACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057003:ENSMUST00000018632:21
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.140 A=NA C2=0.395
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=PU(28.5=64.0)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(41.5=100),PF0157614=Myosin_tail_1=PU(29.1=48.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGCCCTGGATGAAGCTGTAT
R:
CGTCCTCCAGCTTCCTCTTCT
Band lengths:
358-1056
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types