BtaINT0122614 @ bosTau6
Intron Retention
Gene
ENSBTAG00000015537 | RASAL2
Description
RAS protein activator like 2 [Source:HGNC Symbol;Acc:HGNC:9874]
Coordinates
chr16:61289586-61291703:+
Coord C1 exon
chr16:61289586-61289822
Coord A exon
chr16:61289823-61291501
Coord C2 exon
chr16:61291502-61291703
Length
1679 bp
Sequences
Splice sites
5' ss Seq
CAAGTAGGC
5' ss Score
5.3
3' ss Seq
TTTCTCTCCCTCTACTTCAGATT
3' ss Score
10.79
Exon sequences
Seq C1 exon
TGTTTTTCCTCGTGAGCTGAAAGAAGTGTTTGCATCCTGGAAGCAGCAGTGCCTGAACCGCGGCAAGCAGGACATCAGCGAGAGGCTCATCAGCGCCTCGCTGTTTCTCCGTTTTCTGTGCCCGGCCATCATGTCTCCCAGTCTCTTCAACCTCATGCAGGAGTATCCTGATGACCGCACATCTCGGACTCTGACTCTCATTGCCAAGGTCATCCAGAACCTGGCCAACTTTGCCAA
Seq A exon
GTAGGCGGAGCTACCATAACCACTTTCTCAAAACGTCACTTAAAAGTACATGAGGCTTAACCTTTAGATGAGCATGGGCCACCTCATCACTCATGTTACATGGTTTTCATCGTGATGTTATTAAAAACAGCAAAAAGATTGACATCACTTTGAATATTCACTTTGTAGAGGAATAGTTAAGTAATCCATGCATCCAATGGAATATATGCAGCTACTACAAAATATGTTTACGAATAGTATGGAAAATGATAAAGATGTAATTATATTAAATAAAAAGTGCTGTTTAAAAAAAGAGATAGCCAAAAAGAGAGGACACCAAATGGTGTATATGCTTTTATCCCCCAATATGATATTGCAAAAACCTAAATATAAGAAAGTTGGGAAGGAATTGGCCTGACCCCTTCAAAAAGTCAATATCATGAAAAATGGAAAAGATGAAGTGGGGACTATTCTGGATAAAAGAGACAAGGCAGTGTTTGGATCTGAATTGGAAAAAAATTTATTAAAATCATTTTGGGAATAACTGGTGAAATAGGAACTCTATATGAGATGATTTATAAATAACATTTTCTTAAAGTTTCAGTGTGATAATGGCGTTCGTATGTGTAAGAGTTATTCATTATTCTAGAGAAGTAGGTACTAATGTATTTAGGGGTTATATCTGCAACTTAGTTTAAAATGGTTCAATCAAAAAAAGAGAGACGGTGAAAACAGACTTGGCAAAATGTGAACGGTTGGTTAAGTAGGTAAATGGCATTTGGGATTTGAACTTGAATAGAACCCTGTATATTCTTTGAAATTTTCTGTAGTTGTAAACTTTTTCAAAAAGAAGTTAGGAGAAATCAGCTGGAGAAGAAATGCACTGAACTGTAAACAGCAGATATGTTTGGGTGGTAGAGTTGGTACAGCCATCCTTTTTTCTTTCCACTTTTCAGTGTTTTCAAATTTTTCTTAAGTTTGTGTAATTCTTATAGTTTATACGAAATAATTTTAAGAAATCACTGAGAAGCAGTTCACACTGGCAGAATTGATCCCAGATTCTCTCAATATTGAAGCCAAGTATGTTTCTAGTAGCAGTGTATTGAAGGGAAAAATTAGAACTTCAATGAAACACTGTTTTCTAGCATGTGGCTCTTGGAAAGTGCACGAGTTTAACTTTGTGTGCACAACATATATGCCAGCAGAGGAATCTATGTTTACCCATATTTGATCTAATTCTTATAAAAATTGTTGATAGTTTATTTTAGAACATGTTCTTTGGATTTAAGCTAGGCCCAGAAGATAGCTACAACCTAGACAGACAAAAAAGAGAGGAAATTCTCTAGTAGACATAAATAGCATAAGCAAAGGTTTGGAGAAGAAAAGAAGTATACTATATTTGGGAGATTTTGAAGAAGCTAGTGATACTGAGGTTTATACTGATAATGTTGATACTGGCTTTAAATACCAGGGAAAAAGGACATTGTCTTTACCCTATAGAGAATAGAAAGCTATTCAAAGTTTTTGAGTATTAATGTAATATTAAGAAAGTATGTTTTAATACTTATCACTTACAAATAATAAAACAAATTAGTCACCTCCCTAAAAAAAAAAGTATAGCTCTGTGTAAAGTCAATTCAAAATGATCACACCTCTTTTCCTGAATATGTTCTTTCTCATTTTCTCTCCCTCTACTTCAG
Seq C2 exon
ATTTGGTAACAAGGAGGAATACATGGCATTCATGAATGATTTTTTAGAACATGAATGGGGTGGAATGAAGCGCTTTCTTTTGGAGATCTCTAATCCAGACACCATCTCAAACACCCCAGGCTTTGATGGTTACATTGATCTGGGCCGAGAGCTTTCAGTTTTGCATTCCTTACTATGGGAAGTAGTTTCCCAACTTGATAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000015537:ENSBTAT00000020658:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0061614=RasGAP=PD(44.2=95.0)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGTGTTTGCATCCTGGAAGCA
R:
CCATCAAAGCCTGGGGTGTTT
Band lengths:
342-2021
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development