DreINT0124793 @ danRer10
Intron Retention
Gene
ENSDARG00000036257 | rasal2
Description
RAS protein activator like 2 [Source:ZFIN;Acc:ZDB-GENE-070912-180]
Coordinates
chr2:35856695-35857341:+
Coord C1 exon
chr2:35856695-35856931
Coord A exon
chr2:35856932-35857139
Coord C2 exon
chr2:35857140-35857341
Length
208 bp
Sequences
Splice sites
5' ss Seq
CAAGTATGT
5' ss Score
7.44
3' ss Seq
CTAATTGTCTATTACAACAGATT
3' ss Score
5.39
Exon sequences
Seq C1 exon
TGTTTTTCCACGTGAACTGAAGGAGGTCTTTGCATCATGGAAGCAGCAGTGTCACGCTCGAGGGAAGCAGGACATCAGTCAGCGTCTTATCAGCGCATCGCTCTTCCTGCGCTTTCTGTGTCCTGCCATCATGTCCCCGTCACTCTTTGGTCTCATGCAGGAATATCCAGATGACCGTACTTCTCGAACACTCACCCTCATTGCCAAAGTCATTCAGAATCTTGCTAACTTTGCCAA
Seq A exon
GTATGTGCCATTATCTCATGCAAGAAACAATGTTTACATGTAATGTTTTCACATTAAATACTGTACTGATTGTGTTACTTAAATTAAATACCATATTAATATTATATTCTTGTGTTAGTAAATGTAGTATTTGTAAAAGTCTATTATCAGTCTGCCTCAGAGCACTTCTAATGCATTCTAATACATTTCTAATTGTCTATTACAACAG
Seq C2 exon
ATTCGGGAACAAAGAGGAGTACATGGCTTTCATGAATGACTTTCTAGAGCACGAGTGGGCTGGTATGACCCGTTTTCTGCTTGAGATCTCGAATCTTGAGACAATCTCCAACACCCCGGGCTTTGAGGGCTACATTGACCTGGGCCGGGAGCTGTCAGTGCTTCACTCGCTGCTCTGGGAGGTGGTGTCTCAGCTGGACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000036257:ENSDART00000113489:11
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0061614=RasGAP=PD(44.2=95.0),PF067666=Hydrophobin_2=PD(51.0=31.2)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTTTCTGTGTCCTGCCATCA
R:
GAGTGAAGCACTGACAGCTCC
Band lengths:
295-503
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]