BtaINT0124257 @ bosTau6
Intron Retention
Gene
ENSBTAG00000013645 | RFFL
Description
ring finger and FYVE-like domain containing E3 ubiquitin protein ligase [Source:HGNC Symbol;Acc:HGNC:24821]
Coordinates
chr19:15395107-15396292:+
Coord C1 exon
chr19:15395107-15395190
Coord A exon
chr19:15395191-15396081
Coord C2 exon
chr19:15396082-15396292
Length
891 bp
Sequences
Splice sites
5' ss Seq
CAGGTGGGC
5' ss Score
8.07
3' ss Seq
TCCCATCTTGGCCTTCGCAGTCC
3' ss Score
8.03
Exon sequences
Seq C1 exon
GCCAATGGCCATGTGTCTCAGGACCAAGAGGAGCCCATCTACCTGGAGCGCACAGCCAGAGCCCCTGCTGAGGACGAGACCCAG
Seq A exon
GTGGGCCCTGTGGGTCTGTGTGTCTGCCTTTGCTCTCACTGCTGTTCTTGTCAGGACCCTCTGGGCAGCCTCACGGGGGAGAGGGGAGGAGCCGGCTGTGATGACAGTCCCGAAGTCCTGTCTGCGGGGGTGGCGTTTAGTGTGAGTGTTCAGGTGAACCTCAGGAGCCGTCCGTGTCCCAGTGGAGGCTGCGGAGCGGCACAGCCCGTTGGTTTCCAGTCTGCTGACTTGGAGAGCAGCCAGAGGGCAGGCTCCTGGTGCCGCTGTGCCAGTCTTGTTCTTTTCCTCTGCTTCCAGCACACAGTGGCCATTGTCAGGAGAAGGAGGTCAGATAATCAGGAGGAAAGATGAGAGGGAGTTCAGGGCACTGCCGTGGGGCCGCAGAGCTGTGGTCTCCAGTGTGCAGAGAGGATGCTTGCGCGGGAAGCAGGCTTCTTCTGTGGGAAAGCAGAACTCTCTCCGGGGGAGAAAGACCCGGACCACGCACGGTAGTCTCTTGTGCTGCCTCGTTTAATCTCCATGGGAAGCCTGGGGCACACGGGCCCAGAGAGGTGAGCATCGTGCCCGGTGCTGCTCAGCATCAGTCAGTAGAGCAATCGGGATCCAAAACCCCCTGCCTTAGCTGGTGCTGCTCCTCCCGCCTGGCCACTGGGGGTGGGGCTGTGGCCCTCTGGGCCTGAGCACAGCCCTTGGGTCAGGAAGAGACGGAGGTCTGTCTTGCGATGTAGAGCCGGGGCCACGTAATCCTCCATGTGCCCCCATTCCTGCTTCCCTTGAGGCCAGTCACCAAAAGCACAGGGTTTTGAGAGCTCTGGGGTCCTGGGAGCGAGAGCCGGGGCGGGGGCGGGGGCGGGGGAGTGGAGCCCACCCATCCCATCTTGGCCTTCGCAG
Seq C2 exon
TCCGTCGACTCAGAGGACAGCTTTGTCCCAGGCCGGAGGGCCTCTCTGTCTGACCTGACCGACCTGGAGGACATCGAAGGTCTCACCGTGCGACAGCTCAAGGAGATCCTGGCTCGCAACTTCGTCAACTACAAGGGCTGCTGTGAGAAGTGGGAACTGATGGAGAGGGTGACGCGGCTGTACAAGGACCAGAAGGGCCTCCAGCACCTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000013645:ENSBTAT00000018136:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=NA C2=0.254
Domain overlap (PFAM):
C1:
PF102084=Armet=PU(12.3=35.7)
A:
NA
C2:
PF102084=Armet=PD(85.2=97.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCCAATGGCCATGTGTCTCAG
R:
CAGGTGCTGGAGGCCCTTC
Band lengths:
294-1185
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]