BtaINT0124449 @ bosTau6
Intron Retention
Gene
ENSBTAG00000016827 | RGL2
Description
Bos taurus ral guanine nucleotide dissociation stimulator-like 2 (RGL2), mRNA. [Source:RefSeq mRNA;Acc:NM_001075154]
Coordinates
chr23:7412190-7412626:-
Coord C1 exon
chr23:7412576-7412626
Coord A exon
chr23:7412488-7412575
Coord C2 exon
chr23:7412190-7412487
Length
88 bp
Sequences
Splice sites
5' ss Seq
AGGGTAAGT
5' ss Score
10.45
3' ss Seq
GTGAATTCCCCTTCTCCCAGGGT
3' ss Score
7.67
Exon sequences
Seq C1 exon
GCTGGAAGCTCTTGAATCTCATCCTGCTGATGAGCTAGAGAGGACAAAAGG
Seq A exon
GTAAGTGACCCCTGAGTCTGACTTCACAGTTTTCTCCGGCCAAGCAGCAGCCTGACACCTAATCCCCGGTGAATTCCCCTTCTCCCAG
Seq C2 exon
GGTAGCCATCTCTGTACTGTCAACCTGGCTGGCCTCTCACCCTGAGGATTTTGGCTCTGAGGTCAAGGGTCAGCTTGACCGGCTTGAGAGCTTCTTTCTTCGGACAGGGTATGCAGCAGGGGAGGGTATTGTGGGGGGCGGCGCTGACCTCATCCGCAACCTCCGGTCCCGGGTGGACCCCCAGACCCCCGAACTTCCTAAGCCCTTGGCCCTCCCCGGCGATCCCCCTGCTGACCCCACGGATGTCCTGGTGTACCTCGCTGACCACTTGGCCGAACAGCTGACCCTGCTAGATGCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000016827:ENSBTAT00000022385:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.278 A=NA C2=0.180
Domain overlap (PFAM):
C1:
PF0061815=RasGEF_N=FE(17.3=100)
A:
NA
C2:
PF0061815=RasGEF_N=PD(31.6=31.0),PF0061714=RasGEF=PU(7.2=16.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Chicken
(galGal4)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCTCTTGAATCTCATCCTGCTG
R:
AATACCCTCCCCTGCTGCATA
Band lengths:
175-263
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]