BtaINT0124450 @ bosTau6
Intron Retention
Gene
ENSBTAG00000016827 | RGL2
Description
Bos taurus ral guanine nucleotide dissociation stimulator-like 2 (RGL2), mRNA. [Source:RefSeq mRNA;Acc:NM_001075154]
Coordinates
chr23:7411668-7412487:-
Coord C1 exon
chr23:7412190-7412487
Coord A exon
chr23:7411920-7412189
Coord C2 exon
chr23:7411668-7411919
Length
270 bp
Sequences
Splice sites
5' ss Seq
GCGGTGAGA
5' ss Score
7.4
3' ss Seq
CTCTTACCTCTGCCCTGCAGGAG
3' ss Score
9.43
Exon sequences
Seq C1 exon
GGTAGCCATCTCTGTACTGTCAACCTGGCTGGCCTCTCACCCTGAGGATTTTGGCTCTGAGGTCAAGGGTCAGCTTGACCGGCTTGAGAGCTTCTTTCTTCGGACAGGGTATGCAGCAGGGGAGGGTATTGTGGGGGGCGGCGCTGACCTCATCCGCAACCTCCGGTCCCGGGTGGACCCCCAGACCCCCGAACTTCCTAAGCCCTTGGCCCTCCCCGGCGATCCCCCTGCTGACCCCACGGATGTCCTGGTGTACCTCGCTGACCACTTGGCCGAACAGCTGACCCTGCTAGATGCG
Seq A exon
GTGAGACCCTGACCTCTGACCCCATGACCCCTGCCCCCTTACTAACCTGTCCCTAACTCCACATCCTTACCTGGCTTCCAGTCCTTCCTGGTCCAGCTCCCAGCCTCCTCTGTCCACCCAGTTTTGACCTTTCCCCATTTCCCCTTGATTGTTGCCCTTCAAACCCTATGCCCCTCTGGTTCCTTGGCCACATGAGATCCTCACACCCCTGACATTGGAAGGCTGACCTCACTTGACTATGAACTTTAACCTCTTACCTCTGCCCTGCAG
Seq C2 exon
GAGCTGTTTCTCAATCTGGTCCCCTCTCAGTGCCTGGGGGGCCTGTGGGGTCACAGAGACCGGCCAGGACATTCTCACCTCTGCCCATCTGTCCGGGCGACTGTCACACAGTTCAACAAGGTGGCAGGGGCAGTGGTCAGCTCTGTCCTGGGGGCTACCTCAACTGGAGAAGGGCTTGGAGATGTGACCGTACGGCCACTTCGTCCTCCCCAGAGGGCCCGGCTCTTGGAGAAGTGGATCCGCGTGGCAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000016827:ENSBTAT00000022385:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.180 A=NA C2=0.083
Domain overlap (PFAM):
C1:
PF0061815=RasGEF_N=PD(31.6=31.0),PF0061714=RasGEF=PU(7.2=16.0)
A:
NA
C2:
PF0061714=RasGEF=FE(37.2=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Chicken
(galGal4)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCAGACCCCCGAACTTCCTAA
R:
TCCAAGCCCTTCTCCAGTTGA
Band lengths:
298-568
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]