BtaINT0155283 @ bosTau6
Intron Retention
Gene
ENSBTAG00000046886 | TUBB2B
Description
Bos taurus tubulin, beta 2B class IIb (TUBB2B), mRNA. [Source:RefSeq mRNA;Acc:NM_001003900]
Coordinates
chr23:50391768-50392323:+
Coord C1 exon
chr23:50391768-50391876
Coord A exon
chr23:50391877-50392212
Coord C2 exon
chr23:50392213-50392323
Length
336 bp
Sequences
Splice sites
5' ss Seq
CTGGTGAGG
5' ss Score
8.3
3' ss Seq
CTCCTGCTGTGTCCCCGCAGGTA
3' ss Score
13.95
Exon sequences
Seq C1 exon
TTTTGGGAAGTCATCAGCGATGAGCATGGCATCGACCCCACTGGCAGTTACCATGGAGACAGTGACTTGCAGCTGGAGAGAATCAACGTGTACTACAACGAAGCTGCTG
Seq A exon
GTGAGGACTGCCTCGTGCTCGGCCCCCTCTCCTGTGTTCCGTTCTCTGGCAGCCCCAGTGTAGCAGGAACACACACACACACACGACAAAGAAATGTTCCCTGCATGCGTTTAAAGTCAGCTGCTCAGAAATACCCTCAGAGTCCCTGAAGTTCCCTGCATTCGGGTCATGACCGGGCAGGCCCTCACCTCCCTTTGTAGAGCAGAAACCTCCTCTAATCTCTTTGCGTCTTGCTGGAATTTTCTGGGATGAGCTCTGCCTGGAGGGGCACGGAGAGCAGAGTGTTGTCTGATTGCTCTGCAAGCGCCAGGTCTGACTCCTGCTGTGTCCCCGCAG
Seq C2 exon
GTAACAAGTATGTGCCTCGGGCCATCCTGGTGGACCTGGAGCCGGGCACCATGGACTCCGTCAGGTCTGGACCCTTCGGCCAGATCTTCAGGCCCGACAACTTCGTGTTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000046886:ENSBTAT00000044257:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.108 A=NA C2=0.026
Domain overlap (PFAM):
C1:
PF0009120=Tubulin=FE(16.2=100)
A:
NA
C2:
PF0009120=Tubulin=FE(16.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTTTGGGAAGTCATCAGCGATGA
R:
CGAAGTTGTCGGGCCTGAAG
Band lengths:
214-550
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]