BtaINT0155288 @ bosTau6
Intron Retention
Gene
ENSBTAG00000021013 | TUBB4A
Description
Bos taurus tubulin, beta 4A class IVa (TUBB4A), mRNA. [Source:RefSeq mRNA;Acc:NM_001034697]
Coordinates
chr7:19206710-19207038:+
Coord C1 exon
chr7:19206710-19206818
Coord A exon
chr7:19206819-19206927
Coord C2 exon
chr7:19206928-19207038
Length
109 bp
Sequences
Splice sites
5' ss Seq
CTGGTAAGG
5' ss Score
9.68
3' ss Seq
GTCCATCTGCTTCTCTGCAGGAG
3' ss Score
9.56
Exon sequences
Seq C1 exon
TTTTGGGAGGTAATCAGTGATGAGCATGGCATTGATCCCACGGGCACATACCACGGGGACAGTGACCTCCAGCTGGAGAGAATCAACGTATACTACAATGAGGCCACTG
Seq A exon
GTAAGGGGGCAGAGGCACCTGGCCAGAGTGGGGGTGGAGAGGGCCTGGGAGGGGACTGGGTGCTGGTTCCTGGAGTTGACCAAGCTGCTGTCCATCTGCTTCTCTGCAG
Seq C2 exon
GAGGAAATTACGTGCCCAGAGCTGTCCTGGTGGACCTTGAGCCAGGCACCATGGACTCTGTCCGCTCTGGCCCCTTCGGCCAGATCTTTCGTCCTGACAATTTTGTGTTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021013:ENSBTAT00000014560:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.162 A=NA C2=0.026
Domain overlap (PFAM):
C1:
PF0009120=Tubulin=FE(16.2=100)
A:
NA
C2:
PF0009120=Tubulin=FE(16.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTGGGAGGTAATCAGTGATGAGC
R:
CAAACACAAAATTGTCAGGACGA
Band lengths:
218-327
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]