BtaINT0165507 @ bosTau6
Intron Retention
Gene
ENSBTAG00000046301 | ZNF445
Description
zinc finger protein 445 [Source:HGNC Symbol;Acc:HGNC:21018]
Coordinates
chr22:16367996-16371191:-
Coord C1 exon
chr22:16371081-16371191
Coord A exon
chr22:16370167-16371080
Coord C2 exon
chr22:16367996-16370166
Length
914 bp
Sequences
Splice sites
5' ss Seq
CAGGTGAGT
5' ss Score
10.67
3' ss Seq
TTATGTTTTATTTGTGTCAGGAG
3' ss Score
6.05
Exon sequences
Seq C1 exon
TGGGGCCATTCACCAAACCTGCTCTCATCTCCTGGCTGGAGGCAAGGGAGCCATGGGGCCTGAATGTCCAGGGAGTTCAGTTTAAGGGGAATCCAGGTGCTGCCCCTGCAG
Seq A exon
GTGAGTTCCAGAGAAATGACCAAGTCTTGCTCCTTTCACATCTGCTCTTCTGTTTCTTAAGGTCTTTTACACTACGAAAATCGTGGGAGACTCTTTCTGTTGTTTGCTAGAAGGAAGAGCCTTAGCAGTGTTGCTAATAAATGTTGGGTGCTTGGGCTTCTCCCCTCCAGAGTGGCCTCTTAGATGTTGGTTTTCAGCCTTTCCCCTCACCCAGAAGCCCTCTCAGGAGCAGCTCTTGCCCACAGGACAGCATCACTGTCATCTTGTCCATTCACTGATGCTCCTCCTCAGCCCTGTACTGCTCTGCCCTTCCTGCTCCTGGCACCCCCTCATCCCACTTTTGCTGCTGTTCTTTGCTTGGTCCTTCCCCTGTGGTCAGCTTGCAGCCCTTGCAGCCAGCTCTCCTCAAGCCCTTCCTCTCAGCTCTTGAGGACCTTCCTGTCATGTGTGCGTGTCATGCTTCCTCTCCCCTCTCTTGCTGTTTAACACTCCCTTCTCAACCCTTTCGGTTTTCCCCTGGATTTGATCCCTGCATGTGGCCAGCTGGTGTACCCATCTTGCTTCTGCTCTCCCCTTTCTGTCATCTGGAATACCCCTCCATGTTTTGCCTTATCCCTGACTGTTTTTGTAAAGCTACTGGCAGTCAGGCAAAAGCCTCACCACCTAAGTTCATGACATTTTTTGGTCCCTTGGTGCTTTCTGTATTTCCAAGTATGGGAAAAGAGAACATTCTTTAGTCCCCCCCCCTCCGCCCCCACCTTTTTTTTTAAACCACACCTAGTGGCTTATGGGATCTCAGTCCCCTTACCAGGGATTGAACGTGGGCCATGGCAGTGAAAGCCTGGAATTCTAACCACTGGGCAACCAGGGCACTCCCAAGAATAGTACATTCTCTTATGTTTTATTTGTGTCAG
Seq C2 exon
GAGATGAGCTCCATGTGAAAACAGACAAGTTCATCTTAAAACCTGAACCTTTGGAAGAAGCAGTAACCTTAGCCATGCCATCAGGATGTCATGGTGCAGCGACTGTTTCTGAGGGACCAGGGCTCAGAGAATCCATTGCACAGAAGAGTGGGTTAAAGGAACGATGTGGAAACCCCACACAAGTGAGAGTTAAGAAAGAAGAGACCAATTCCAGCCATGGGGCAGGGAAAGACTGCGAAGATTCAGGAAGAAGTAATAGTCTTCATCTAAGACATATTACGTATTTAAGAGGACCCAGAAGAAAGCAGTCCCTTAAACATGGCTATGGCAAACACTTCAGGGGAAGTTCCTACCACTATGACTATAAGGAGTATGGGAAAGGGCTCAGACGCATGATGGGTGGGTTCAGCCTACATCAGAGAATTCATGCTGGACTGAAAGGCAAGGCCAAGGATGCGCATGGGAAGGACTTCAGCCTTAGTTCCCATCACCAACACGGGCAGAATCTTCACGTGGTGGGGACGCTGTATAAGTGCAGCGACTGCGGGAGGACCTTCAGTCGTAGCTCCCACCTTGTATGTCATCAGAGACTCCACACTCAAGAGAAGCCTTTTAAATGCAGGGCATGTGGGAAAGCCTTCAGGTGGAGCTCGAACCGGGTGCGACATGAGAAAATTCACACCAGACTGAAACCGTATAAGTGCAGTTTATGTGACAAAGCCTTCCGTCGCATGTCTGTCTACCGCCTGCACCAGGAAACCCATGCTAAGCAGAAATATCTTGAATCTACTCAGGATGAAGAAGCTCTGACCTGTGTTTCAGATTTTGATCATCATTTGAGAGACCAAAGTGGGGAGAGACTCTTTGACTGCAGCCAGTGTAGGAAATCCTTCCACTGTAAATCATACATTCTTGAACATCAAAGGATTCACACCCAGGAGAAACCCTATAAATGTCCCAAATGCAGGAAAACATTTAGGTGGAGGTCCAACTTCACTCGTCACATGAGACTGCACCAGGAAGAGAAGTTCTATGACCTGGACAAATGTAGAGAAGAGTTGAGGCAGACCTCCAACTCCAGCCAGCCCCAGAGCGCCCCCACCATGGAAAAAGCTCTTGTGTGTCAGCAGTGTGGGAAAACTTTCATTAGAAAGAAGACTCTCATCAATCATCAGAGAATTCACACAGGCGAGAAACCATACCGATGCCACGAATGTGCAGAAGAGTTTCCTCACAGGTCGGCCTACATTGTTCATAAGAGGCAGCATGCCACCAAAAGAAAACCTGAGGACGCACCCTCGTTTAGTCATGATAGAGCGTTCCAAGTTCCTCAGACCAGTCCCACCACAGAGGAGCCCTACAAATGCAGCCAGTGTGGCAAAGTCTTCCGCAATCATTCGTTCCTCCTCATCCATCAGAGAGTTCACACCAGAGAGAAGCCTTATAAGTGCAAAGAGTGTGGGAAATCTTTCAGATGGAGTTCTAACCTCTCCCGACATCAGAGGACTCACTCTTTGGGAAAACTGGATGAGTACTCCGAAAGCGAAGATGCTCTAAATCTTCAGTCACAAGTCCTCACTGGTGCAAAACCTTTTCAGTGCCAAGAATGTGGGAAAAACTTTACACGTAAAAGAAGTCTTTTAGATCATGAGGGAATACACAGTGGAGAGAAGCGCTATAAATGTAACCTGTGTGGGAAATCTTATGACAGAAATTATCGCCTTGTTAATCATCAGAGAATCCACACTAAAGAGAGACCGTTTAAATGTCAGTGCTGTGGGAAAGATTTCATTGGAAAACATACCCTCTCCATTCATCAGAGAAAACACATCAGAGCAGCCCAGTCTGAAAATGGTGTGGCTGGGCTGTCTTCCTGCCAGGAGACAGGGGTAAAGTTACATGAATTAAAATCAGGTCAGGAGAATCCTCTTGAAGATGATGAGAAAACCTGTAATCAGAGATCTGAACTCCCTGGACTCCAGGACATACCCACTCGGGGAAAGTGCCACAAATGTAGCACATGTGGGAAAACTTTTAGCAAGAGCTCACAACTCATCAGCCACAAGAGATTCCATACTCGGGAGAGGCCCTTCAAATGCAGAGTGTGTGGGAAGACCTTTAGGTGGTCTTCAAACTTGGCTCGGCATATGAAAAACCATATTAGAGATTAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000046301:ENSBTAT00000065626:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.421 A=NA C2=0.259
Domain overlap (PFAM):
C1:
PF0135222=KRAB=PD(0.1=0.0)
A:
NA
C2:
PF0009621=zf-C2H2=WD(100=3.2),PF134651=zf-H2C2_2=WD(100=3.6),PF134651=zf-H2C2_2=WD(100=3.6),PF134651=zf-H2C2_2=WD(100=3.3),PF0009621=zf-C2H2=WD(100=3.2),PF139121=zf-C2H2_6=WD(100=3.7),PF134651=zf-H2C2_2=WD(100=3.3),PF134651=zf-H2C2_2=WD(100=6.6),PF134651=zf-H2C2_2=WD(100=3.6),PF134651=zf-H2C2_2=WD(100=3.5),PF139121=zf-C2H2_6=WD(100=3.6),PF0009621=zf-C2H2=WD(100=3.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TAAGGGGAATCCAGGTGCTGC
R:
AGGGACTGCTTTCTTCTGGGT
Band lengths:
342-1256
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]