RnoINT0168231 @ rn6
Intron Retention
Gene
ENSRNOG00000025554 | Zfp445
Description
zinc finger protein 445 [Source:RGD Symbol;Acc:1559144]
Coordinates
chr8:131888047-131890964:-
Coord C1 exon
chr8:131890854-131890964
Coord A exon
chr8:131890113-131890853
Coord C2 exon
chr8:131888047-131890112
Length
741 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAGC
5' ss Score
9.6
3' ss Seq
TGTTTCTTGTTTTCCCTCAGGAG
3' ss Score
11.1
Exon sequences
Seq C1 exon
TGGGCTCTTCTCCCAAACCTGCTCTGATCTCCTGGCTGGAAGCAAGGAAGCCGTGGGGCATGAATATCTGCACAGTTCAGCTTAAGAGGGAACCTGATGCTGCCCCTGAAG
Seq A exon
GTGAGCTCGGTCGAACCTACCACATCATTCCCCTCTTACTTTTGTTCCTTTCTTTCATATGGTTAAATATTTCACTGGGGACTTACTCCATTATTTGCCAGGAGTTACTAAGGGATGCTATGGACCTGGGGCTCTTCCTGCTTGGCTTATAATAGGCCATTTCTTTCCAGGCTCGACCATTCCGAGGCGACTCAGTGTCAGTTCTTGCTCATCACAGGGCTTCTCTGTTCTCTTCCCACTGAGCTCTAATTCATGTCTGTGCCGCGCTAAGTCTGAATTGTTCATCTACCCGTTCTGGTTCTTCTTCCTTGTTTGGTTCTCCTTGTGTGATGAGCTTGAGATTTAGTTTTTTTTCAGCAAGGCCCTACTTAGAGCTCCAGGTCTTGGTTTTTGGGGACTTCTCTGTGGTGCTTCTGTGCCATAATTTTTCTTTATTGCTATTAATATTCTTCTTTAGGTCTGTTTATTGCCCTGCTAGATTTGATTTCTGTGTCCCTCCCTCTACCTGCTGTATCTGGCCCTCTCTCCTCCCTTTCCTGTTATTTGAGATACTCTTTCTTTGTCTTATCCTTAACTATTTTGTATAAAGTAATGACAGTCAGGCAAAATTCTTGCTACCAAGAATCATATTTATACTTCCTTGTTGCCTTTTCCAGTTCCAAATATGGAAGAAAAATACACTTTTTTGTGTTGTGTGGGTGCGTGTGTGTACATGTGTTTTTGTTTCTTGTTTTCCCTCAG
Seq C2 exon
GAGGTAAGCTTCAAATTGAGCCAAGCAAGTTCATCTTGAAACAGGAACCTTCAAAATACACAGAAACCTGTGTAAAGCCATCAGGATGTCCTGCTTCTAAGGGAACAGGACTCAAAGAATCTTTTGAACAGAAGAGTAGGTTACAAAAGTCCTGTGGGGATTCCGTACAAATGAAAGAGATGAAGAAAGGAACTGACATTAGTCAAAAGGCAGGAAGAAAATCTGAGATACTGAGAAACAGTGATATTCTTGAGTTAAAACATGTTAAGTGTGTGAGTGTTTCTAGGAAAAAGCTATCCTTTAAACATGGCTATGACAGAAACTTCAGAAAAAGTTCACACCATTATAATAATAAATATGGGGAAGGGCTCAGAGACACAGTTGAGGGGTTTGGTGTATATCAGAATACTGGACTGAAAGAGAATGAAAAGGACAGATGTGGGGAGACCTTAAGGAAGAGCTCCCATTCCCATCTTGAATATCAGAGATATTATAGTCAAGGAAGCCTGTTTAAATGCAGGGTGTGTGAGAAAGCCTTCAAGTGGCGATCAAACTGTATCCGACATGAGAAAATTCACACTGGAGTGAAGCCTTACAAATGCAGTTCATGTGAGAAAGCTTTCCAGCGTTTGTCAACCTACCGTCTGCACCAGAAAACCCATACTAAACAGAAACATGAATCCAGGAAGTACAACAATGCTCTCCCCTGCAGCTTGGATGTCAGTCATTGTTTGATAAACCAGGATGAGGAGAAAAACTTTTACTGTAGCCAGTGTGGGAAACACTTCTCCTGTAGGTCCTATGCTCTTGAACACCAAAGGATTCATACACAGGAGAAACCTTATAATTGTATCAGATGTAGGAAAACCTTTCGGTGGAAGTCAAACTTTACTCGTCATATGAAATTACATGGCAAAGAGGTCCGTAATCAAGAGAGATGTCAAGAAGACTTCAAGCAGAATTATAGTCAGTCTCAGGTTATCTCTACTATAGAGAAAACTTTTCTGTGTCAGAGTTGTGGGAAAACTTTTACTCAAAAGAAGTCACTCATTGAGCACCAGAGGATTCATACAGGGGAGAAACTGTACCAGTGTAGTGGGTGTGGGAAAACATTTGCCTCTAGGTCCTCTTATATTATTCATATGAAGCAAAAGCGACATGCTATTAAAATAAAACCTGAAAGTGGCTCTCTACCTTTTAGTCAGGATACAGCATTTGCCATTCCTCAGAGTGGTCATAATACAGAGGAGCCTAATCAGTGTAAATACTGTGGCAGAGCCTTCCATAATCGCTCATTTCTTCTCATTCACGAGAGAATTCACACTAGAGAGAAGCCCTATAAGTGCAGGGAGTGTGAAAAAGCTTTCCGATGGAGGTCCAATCTCTACCGACATGAGAGAAAACACTTTTTGCACAAGCGGCGTAAGTATCATGAAAGTAAAGAGACTTCAAATCTACAGTCAAAAATCTTCATTGATGAGAAGCCCTTTTGGTGTCAAGAATGTGGGAAAACCTTTACACGTAAAAGAAGCCTTTTAGATCATAAGGGAATACACAGTGGAGAGAGACGCTTTAAGTGCAACTTGTGTGAAAAATCTTTTGATAGAAACTATCGTCTTGTTAATCACCAGAGGATCCACACTACAGAGCAACCATTTCAATCTCAGTGGCATGATAAAGACTTTGCTGGGACACATGCCCATTCTGTTGATCAGAGAAAACACAGAACACTGCAGTCTGAATATAGCCTACAATCAGATAAGCCTGGCTTATCCTACTGTCAGGATGTAAGGGTAAATATTCAGGAATTAGAACTAAGTGGAAAGAAGCCCCTTGATAACCCTTCTCATGAGAGTTCCATGTCCACCGCATTCCAGAATGTGCCCACTAAGAAAGCCTGCCACAAATGCAGCACTTGTGGGAAAACATTCAGGAAGCATTCACATCTCATTAGCCACAAGAGATGTCACACAAAAGAAAGGCCCTTCAAGTGCATCGTGTGTGGGAAGACCTTCGGGTGGTCTTCCAATTTGACCAGGCATGTGAAAAGCCATTTTAGAGATTAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000025554:ENSRNOT00000034690:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.132 A=NA C2=0.156
Domain overlap (PFAM):
C1:
PF0135222=KRAB=PD(0.1=0.0)
A:
NA
C2:
PF134651=zf-H2C2_2=WD(100=3.6),PF134651=zf-H2C2_2=WD(100=3.3),PF134651=zf-H2C2_2=WD(100=3.8),PF134651=zf-H2C2_2=WD(100=3.5),PF134651=zf-H2C2_2=WD(100=3.8),PF134651=zf-H2C2_2=WD(100=3.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGTGGGGCATGAATATCTGCA
R:
GCTTTTTCCTAGAAACACTCACACAC
Band lengths:
354-1095
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]